Natramune and PureWay-C reduce xenobiotic-induced human T-cell α5β1 integrin-mediated adhesion to fibronectin

Background: In vitro and in vivo studies demonstrate that nutritional supplementation reduces inflammation and inflammatory markers associated with T-cell adhesion mechanisms. Here, we investigate the effects of the nutritional supplements, Natramune (PDS-2865) and PureWay-C, on xenobiotic-induced alpha 5 beta 1 integrin-mediated T-cell adhesion to fibronectin. Material/Methods: The human CD4+ lymphoblastoid cell line CEM SS was treated with combinations of bifenthrin, blocking antibodies to human P I and 00 integrin, and nutrient supplements. After 30 minutes unattached cells were aspirated and the percent of attached cells was determined. Results: Bifenthrin Stimulated T-cell adhesion to fibronectin at concentrations between 1.0 and 100 mu M with a maximal stimulation of 8.3-fold at 10 mu M. At 500 mu g/ml, Natramune reduced 100 mu M bifenthrin-induced adhesion by nearly 90%. PureWay-C reduced by 1.5-fold the level of T-cell adhesion stimulated by bifenthrin concentrations of both 10 mu M and 100 mu M. The combination of Natramune and PureWay-C resulted in a 6.3 and 7.5-fold inhibition at 10 mu M and 100 mu M bifenthrin respectively. Antibody blocking studies demonstrated that bifenthrin induced CEM SS adhesion to fibronectin is mediated through alpha 5 beta 1 integrin. Inhibition of T-cell adhesion achieved by anti-integrin antibodies was further reduced with 50 and 500 mu g/ml Natramune treatment. Pretreatment of fibronectin with Natramune did not alter induced T-cell adhesion to fibronectin. Conclusions: These data demonstrate that xenobiotic-induced (601 integrin mediated T-cell adhesion to fibronectin is reduced by nutritional supplementation with Natramune (PDS-2865) and PureWay-C. These data suggest the possibility that inflammatory responses associated with exposure to pollutants can be mitigated by nutritional supplementation.