DNA biosensor for the detection of hydrazines

被引：154

作者：

Wang, J

Chicharro, M

Rivas, G

Cai, XH

Dontha, N

Farias, PAM

Shiraishi, H

机构：

[1] Dept. of Chemistry and Biochemistry, New Mexico State University, Las Cruces

[2] Dept. Analyt. Chem. Instrumental A., Universidad Autonoma de Madrid, Madrid

[3] Department of Physical Chemistry, Universidad Nacional de Cordoba, Cordoba

[4] Department of Chemistry, Pont. Universidad Catolica do RJ, Rio de Janeiro

[5] Department of Chemistry, Ritsumeikan University, Kusatsu

来源：

ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 13期

关键词：

D O I：

10.1021/ac9600619

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A double-stranded (ds) DNA-coated carbon paste electrode is employed as a remarkably sensitive biosensor for the detection of hydrazine compounds. The sensor relies on monitoring changes in the intrinsic anodic response of the surface-confined DNA resulting from its interaction with hydrazine compounds and requires no label or indicator. Short reaction times (1-10 min) are sufficient for monitoring part-per-billion levels of different hydrazines. Applicability to untreated natural water samples is illustrated. The response mechanism is discussed, along with prospects of using DNA biosensors for quantitaing other important molecules and elucidating DNA interactions and damage.

引用

页码：2251 / 2254

页数：4

共 13 条

[1] METHYLATION OF DNA GUANINE DURING THE COURSE OF INDUCTION OF LIVER-CANCER IN HAMSTERS BY HYDRAZINE OR DIMETHYLNITROSAMINE [J].