Characterization of a 2-Cys peroxiredoxin IV in Marsupenaeus japonicus (kuruma shrimp) and its role in the anti-viral immunity

被引：17

作者：

Chen, Xiao-Wei ^{[1
,2
]}

Kang, Li-Hua ^{[1
,2
]}

Ding, Ding ^{[1
,2
]}

Liu, Qian ^{[1
,2
]}

Wang, Jin-Xing ^{[1
,2
]}

Kang, Cui-Jie ^{[1
,2
]}

机构：

[1] Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Minist Educ, Jinan 250100, Shandong, Peoples R China

[2] Shandong Univ, Sch Life Sci, Shandong Prov Key Lab Anim Cells & Dev Biol, Jinan 250100, Shandong, Peoples R China

来源：

FISH & SHELLFISH IMMUNOLOGY | 2013年 / 35卷 / 06期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Peroxiredoxin; Antioxidant; Hydrogen peroxide (H2O2); White spot syndrome virus (WSSV); Antivirus; NF-KAPPA-B; OXIDATIVE STRESS; SERINE-PROTEASE; ANTIOXIDANT; CLONING; INFECTION; PROTEINS; PROTECTS; CLEAVAGE; FAMILY;

D O I：

10.1016/j.fsi.2013.09.018

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Accumulating evidence suggests that peroxiredoxins (Prx) are key molecules in the pathogenesis of various infectious diseases and are potential therapeutic targets for major diseases such as cancers. In this study, we report a peroxiredoxin IV (Prx IV) in Marsupenaeus japonicus, designated as MjPrx IV, which exhibited peroxidase activity and participated in the anti-white spot syndrome virus (WSSV) immune response. MjPrx IV is a 245-amino acid polypeptide with a predicted 19-amino acid signal peptide, an Ahpc-TSA domain, and a 1-Cys PrxC domain. Phylogenetic analysis revealed that the protein belongs to the Pix IV subfamily. MjPrx IV transcripts were detected in the gills, hepatopancreas, heart, stomach, ovaries, spermary, and intestine tissues, and are upregulated in the gonads, gills and hemocytes of shrimp after WSSV challenge. The mature MjPrx IV peptide was recombinantly expressed in an Escherichia coli system. The protein exhibited peroxidase activity. Furthermore, dsRNA suppression of MjPrx IV increased WSSV replication in shrimp, whereas rMjPrx IV injection into shrimp decreased WSSV replication. These data suggest that MjPrx IV has an important role in shrimp antiviral immunity. To our knowledge, this study is the first to report a shrimp Prx IV that has anti-WSSV activity. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：1848 / 1857

页数：10

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