Effects of multicopper oxidase orientation in multiwalled carbon nanotube biocathodes on direct electron transfer

被引：9

作者：

Takamura, Eiichiro ^{[1
]}

Nakamura, Takuto ^{[2
]}

Sakamoto, Hiroaki ^{[2
]}

Satomura, Takenori ^{[3
]}

Sakuraba, Haruhiko ^{[4
]}

Ohshima, Toshihisa ^{[5
]}

Suye, Shin-ichiro ^{[2
,3
]}

机构：

[1] Univ Fukui, Dept Adv Interdisciplinary Sci & Technol, Grad Sch Engn, Fukui, Japan

[2] Univ Fukui, Dept Frontier Fiber Technol & Sci, Grad Sch Engn, Fukui, Japan

[3] Univ Fukui, Dept Appl Chem & Biotechnol, Grad Sch Engn, Bunkyo 3-9-1, Fukui 9108507, Japan

[4] Kagawa Univ, Fac Agr, Dept Appl Biol Sci, Takamatsu, Kagawa, Japan

[5] Osaka Inst Technol, Dept Biomed Engn, Fac Engn, Osaka, Japan

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2019年 / 66卷 / 02期

基金：

日本学术振兴会;

关键词：

biofuel cell; carbon nanotube; direct electron transfer; hyperthermophilic enzyme; multicopper oxidase; oriented immobilization; PYROBACULUM-AEROPHILUM; CONSTRUCTION; ARCHAEON;

D O I：

10.1002/bab.1710

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, multicopper oxidase (MCO) was immobilized on multiwalled carbon nanotubes (MWCNTs) at two different orientations, and the electrochemical properties of the resulting cathodes were investigated. Using N- or C-terminal His-tagged MCO and MWCNTs, we constructed two types of cathodes. We assumed that the distance between the type 1 (T1)Cu of the C-terminal His-tagged MCO and the MWCNT surface was lesser than that between the T1Cu of the N-terminal His-tagged MCO and the MWCNT surface. In addition, in the C-terminal His-tagged MCO, T1Cu was expected to be closer to the MWCNT surface than the type 2/type 3 Cu site. The current density of the modified electrode with a C-terminal His-tagged MCO immobilized on an MWCNT surface was 1.3-fold higher than that of the electrode with an N-terminal His-tagged MCO immobilized on an MWCNT surface. In addition, the amount of H2O2 produced by the N-terminal His-tagged MCO immobilized MWCNT modified electrodes was 2.3-fold higher than that produced by the C-terminal His-tagged MCO immobilized MWCNT electrodes. In direct electron transfer (DET)-type biocathodes, both the MCO orientation and the distance between the T1Cu of MCO and the electrode surface are important. The authors succeeded in constructing highly efficient DET-type electrodes.

引用

页码：137 / 141

页数：5

共 18 条

[1] Construction of a biointerface on a carbon nanotube surface for efficient electron transfer [J].