A novel fluorescence-quenching immunochromatographic sensor for detection of the heavy metal chromium

被引：66

作者：

Fu, QiangQiang ^{[1
]}

Tang, Yong ^{[1
]}

Shi, CongYing ^{[1
]}

Zhang, XiaoLi ^{[1
]}

Xiang, JunJian ^{[1
]}

Liu, Xi ^{[1
]}

机构：

[1] Jinan Univ, Dept Bioengn, Guangdong Prov Key Lab Mol Immunol & Antibody Eng, Guangzhou 510632, Guangdong, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2013年 / 49卷

关键词：

Immunochromatographic sensor; Fluorescence quenching; Chromium; Small analytes; Competitive immunoreaction; ENERGY-TRANSFER FRET; GOLD NANOPARTICLES; RAPID DETECTION; ASSAY; SAMPLES; BIOMOLECULES; IMMUNOASSAY; BIOSENSOR; PROBES;

D O I：

10.1016/j.bios.2013.04.048

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel fluorescence quenching immunochromatographic sensor (ICS) was developed for detecting chromium (Cr3+) within 15 min utilizing the fluorescence quenching function of gold nanoparticles (Au-NPs). The sensor performed with a positive readout. When the low concentrations of Cr3+ samples were applied, detection signals of the test line (T line) were quenched, whereas when higher concentration Cr3+ samples (1.56 ng/mL) were applied, the detection signal of the T line appeared. The detection signal intensity of the T line increased with increasing concentrations of Cr3+ . The low detection limit of developed fluorescence quenching ICS was 1.56 ng/mL. The fluorescence quenching ICS has a linear range of detection of Cr3+ comprising between 6.25 ng/mL to 800 ng/mL. The recoveries of the fluorescence quenching ICS to detect Cr3+ in tap water ranged from 94.7% to 101.7%. This result indicated that the developed sensor gave higher sensitivity and reliable reproducibility. It could provide a general detection method for small analyte in water samples. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：399 / 402

页数：4

共 22 条

[1] Luminescent Silica Nanoparticles: Extending the Frontiers of Brightness [J].