Untargeted metabolomics reveals the role of AQP9 in nonalcoholic fatty liver disease in a mice model

被引：16

作者：

Cheng, Quancheng ^{[1
]}

Zhang, Junwei ^{[2
]}

Fang, Jinyu ^{[1
]}

Ding, Huiru ^{[1
]}

Xu, Yiyao ^{[2
]}

Lu, Xin ^{[2
]}

Zhang, Weiguang ^{[1
]}

机构：

[1] Peking Univ Hlth Sci Ctr, Sch Basic Med Sci, Dept Human Anat & Histol & Embryol, Beijing 100191, Peoples R China

[2] Chinese Acad Med Sci & Peking Union Med Coll, Dept Liver Surg, Peking Union Med Coll PUMC Hosp, State Key Lab Complex Severe & Rare Dis, Beijing 100730, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2022年 / 219卷

关键词：

Nonalcoholic fatty liver disease; Aquaporin; 9; Untargeted metabolomic; EXPRESSION; AQUAPORIN-9; CHANNELS; ADIPOSE; NAFLD; RAT;

D O I：

10.1016/j.ijbiomac.2022.08.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies have shown that AQP9 plays an important role in energy metabolism in nonalcoholic fatty liver disease (NAFLD). Recently, metabolomic analyses were used to determine the slight changes in metabolic profiles and helped to understand the disease progression, therapeutic intervention of NAFLD. A mouse model of NAFLD was established with a high-fat diet (HFD), and Aqp9 knockout mice were constructed. Untargeted metabolomics techniques were used to evaluate the potential mechanism of the effect of AQP9 in NAFLD. The results indicated that AQP9 plays a regulatory role in the occurrence of NAFLD. Moreover, a total of 220 candidate biomarkers were screened and identified. Cluster analysis and enrichment analysis of differential metabolites indicated that fatty acid biosynthesis was mainly disturbed when compared against the control group, which was mitigated by knockout of Aqp9. These results show that untargeted metabolomics help to understand the effects of AQP9 in NAFLD.

引用

页码：864 / 875

页数：12

共 36 条

[1] Role of Oxidative Stress in the Pathogenesis of Non-Alcoholic Fatty Liver Disease: Implications for Prevention and Therapy [J].

Arroyave-Ospina, Johanna C. ;

Wu, Zongmei ;

Geng, Yana ;

Moshage, Han .

ANTIOXIDANTS, 2021, 10 (02) :1-25

[2] Distribution and possible roles of aquaporin 9 in the brain [J].

Badaut, J ;

Regli, L .

NEUROSCIENCE, 2004, 129 (04) :971-981

[3] Aquaporin-9 is involved in the lipid-lowering activity of the nutraceutical silybin on hepatocytes through modulation of autophagy and lipid droplets composition [J].

Baldini, Francesca ;

Portincasa, Piero ;

Grasselli, Elena ;

Damonte, Gianluca ;

Salis, Annalisa ;

Bonomo, Michela ;

Florio, Marilina ;

Serale, Nadia ;

Voci, Adriana ;

Gena, Patrizia ;

Vergani, Laura ;

Calamita, Giuseppe .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2020, 1865 (03)

[4] Molecular pathways of nonalcoholic fatty liver disease development and progression [J].

Bessone, Fernando ;

Valeria Razori, Maria ;

Roma, Marcelo G. .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2019, 76 (01) :99-128

[5] NAFLD: A multisystem disease [J].

Byrne, Christopher D. ;

Targher, Giovanni .

JOURNAL OF HEPATOLOGY, 2015, 62 :S47-S64

[6] Knockdown of hepatic aquaglyceroporin-9 alleviates high fat diet-induced non-alcoholic fatty liver disease in rats [J].

Cai, Can ;

Wang, Chuan ;

Ji, Wen ;

Liu, Bo ;

Kang, Yujun ;

Hu, Zaimin ;

Jiang, Zheng .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2013, 15 (03) :550-556

[7] Altered expression and distribution of aquaporin-9 in the liver of rat with obstructive extrahepatic cholestasis [J].

Calamita, Giuseppe ;

Ferri, Domenico ;

Gena, Patrizia ;

Carreras, Flavia I. ;

Liquori, Giuseppa E. ;

Portincasa, Piero ;

Marinelli, Raul A. ;

Svelto, Maria .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2008, 295 (04) :G682-G690

[8] Aquaglyceroporin AQP9: Solute permeation and metabolic control of expression in liver [J].

Carbrey, JM ;

Gorelick-Feldman, DA ;

Kozono, D ;

Praetorius, J ;

Nielsen, S ;

Agre, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (05) :2945-2950

[9] Aquaporin 9 Represents a Novel Target of Chronic Liver Injury That May Antagonize Its Progression by Reducing Lipotoxicity [J].

Cheng, Quancheng ;

Ding, Huiru ;

Fang, Jinyu ;

Fang, Xuan ;

Liu, Huaicun ;

Wang, Jianwei ;

Chen, Chunhua ;

Zhang, Weiguang .

OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2021, 2021

[10] Genetics and epigenetics of NAFLD and NASH: Clinical impact [J].

Eslam, Mohammed ;

Valenti, Luca ;

Romeo, Stefano .

JOURNAL OF HEPATOLOGY, 2018, 68 (02) :268-279

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