Engineering of bone using rhBMP-2-loaded mesoporous silica bioglass and bone marrow stromal cells for oromaxillofacial bone regeneration

被引：21
作者：
Xia, Lunguo [1 ,2 ]
Zeng, Deliang [2 ]
Sun, Xiaojuan [4 ]
Xu, Yuanjin [1 ]
Xu, Lianyi [2 ,3 ]
Ye, Dongxia [2 ]
Zhang, Xiuli [2 ]
Jiang, Xinquan [2 ,3 ]
Zhang, Zhiyuan [1 ,2 ]
机构：
[1] Shanghai Jiao Tong Univ, Peoples Hosp 9, Sch Med, Dept Oral & Maxillofacial Surg, Shanghai 200011, Peoples R China
[2] Shanghai Jiao Tong Univ, Peoples Hosp 9, Shanghai Res Inst Stomatol, Oral Bioengn Lab, Shanghai 200011, Peoples R China
[3] Shanghai Jiao Tong Univ, Peoples Hosp 9, Sch Med, Dept Prosthodont, Shanghai 200011, Peoples R China
[4] Ningxia Med Univ, Gen Hosp, Dept Oral Maxillofacial Surg, Ningxia 750004, Peoples R China
来源：
MICROPOROUS AND MESOPOROUS MATERIALS | 2013年 / 173卷
基金：
中国国家自然科学基金;
关键词：
Mesoporous silica bioglass; rhBMP-2; Bone marrow stromal cells; Tissue engineering; Oromaxillofacial bone regeneration; MAXILLARY SINUS FLOOR; BETA-TRICALCIUM PHOSPHATE; MESENCHYMAL STEM-CELLS; SCAFFOLD; ELEVATION; DIFFERENTIATION; BIOCERAMICS; DEFECTS; RABBITS; PROLIFERATION;
D O I：
10.1016/j.micromeso.2013.02.020
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
In present study, CaO-P2O5-SiO2-system mesoporous silica (MS) scaffolds were synthesized and loaded with recombinant human bone morphogenetic protein-2 (rhBMP-2), while their protein release properties and other characteristics were investigated. Furthermore, rabbit bone marrow stromal cells (bMSCs) were cultured and seeded on rhBMP-2-loaded MS (rhBMP-2/MS) scaffolds. Cell adhesion and proliferation were evaluated by scanning electron microscopy (SEM) and MTT assays, while osteogenic differentiation was measured by ALP activity and real-time PCR analysis on the osteogenic markers of runt-related transcription factor 2 (Runx2), collagen type 1 (COL1), osteocalcin (OCN), and osteopontin (OPN). Finally, twenty-four rabbits received unilateral maxillary sinus floor elevation surgery at each time point (2 and 8 weeks), and randomly filled with one of the following four materials: MS alone; autologous bMSCs/MS complexes; rhBMP-2/MS complexes; or autologous bMSCs/rhBMP-2/MS complexes. New bone formation and mineralization were detected by histological/histomorphometric analysis, and fluorochrome labeling. The results showed that MS scaffolds presented excellent hierarchically large pore and well-ordered mesopore properties; moreover, rhBMP-2/MS scaffolds efficiently released rhBMP-2 in a sustained manner. Furthermore, rhBMP-2/MS scaffolds significantly enhanced the proliferation and osteogenic differentiation of bMSCs. In the maxillary sinus floor elevation experiments, rhBMP-2/MS scaffolds promoted new bone formation and augmented the height of the sinus floor, while the addition of bMSCs further enhanced new bone formation and mineralization. The present study revealed that CaO-P2O5-SiO2-system MS scaffolds could act as drug delivery carriers for rhBMP-2 and could be used to construct tissue-engineered bone with bMSCs for oromaxillofacial bone regeneration. (c) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码：155 / 165
页数：11
相关论文
共 38 条
  • [1] Bone morphogenetic proteins
    Chen, D
    Zhao, M
    Mundy, GR
    [J]. GROWTH FACTORS, 2004, 22 (04) : 233 - 241
  • [2] BONE TISSUE ENGINEERING
    CRANE, GM
    ISHAUG, SL
    MIKOS, AG
    [J]. NATURE MEDICINE, 1995, 1 (12) : 1322 - 1324
  • [3] Osteogenic evaluation of calcium/magnesium-doped mesoporous silica scaffold with incorporation of rhBMP-2 by synchrotron radiation-based μCT
    Dai, Chenglong
    Guo, Han
    Lu, Jingxiong
    Shi, Jianlin
    Wei, Jie
    Liu, Changsheng
    [J]. BIOMATERIALS, 2011, 32 (33) : 8506 - 8517
  • [4] Tissue engineering for bone defect heating: An update on a multi-component approach
    Drosse, Inga
    Volkmer, Elias
    Capanna, Rodolfo
    De Biase, Pietro
    Mutschler, Wolf
    Schieker, Matthias
    [J]. INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED, 2008, 39 : S9 - S20
  • [5] Bone reconstruction: from bioceramics to tissue engineering
    El-Ghannam, A
    [J]. EXPERT REVIEW OF MEDICAL DEVICES, 2005, 2 (01) : 87 - 101
  • [6] Regeneration of large bone defects in sheep using bone marrow stromal cells
    Giannoni, P.
    Mastrogiacomo, M.
    Alini, M.
    Pearce, S. G.
    Corsi, A.
    Santolini, F.
    Muraglia, A.
    Bianco, P.
    Cancedda, R.
    [J]. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2008, 2 (05) : 253 - 262
  • [7] Bone-graft substitutes: Facts, fictions, and applications
    Greenwald, AS
    Boden, SD
    Goldberg, VM
    Khan, Y
    Laurencin, CT
    Rosier, RN
    [J]. JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2001, 83A : 98 - 103
  • [8] Scaffolds in tissue engineering bone and cartilage
    Hutmacher, DW
    [J]. BIOMATERIALS, 2000, 21 (24) : 2529 - 2543
  • [9] Simultaneous implant placement and bone regeneration around dental implants using tissue-engineered bone with fibrin glue, mesenchymal stem cells and platelet-rich plasma
    Ito, Kenji
    Yamada, Yoichi
    Naiki, Takahito
    Ueda, Minoru
    [J]. CLINICAL ORAL IMPLANTS RESEARCH, 2006, 17 (05) : 579 - 586
  • [10] Enhancement of ectopic bone formation by bone morphogenetic protein-2 released from a heparin-conjugated poly(L-lactic-co-glycolic acid) scaffold
    Jeon, Oju
    Song, Su Jin
    Kang, Sun-Woong
    Putnam, Andrew J.
    Kim, Byung-Soo
    [J]. BIOMATERIALS, 2007, 28 (17) : 2763 - 2771
1234