Bruchid resistance of transgenic azuki bean expressing seed alpha-amylase inhibitor of common bean

Various species of bruchid beetles including Callosobruchus chinensis, C. maculatus and C. analis cause postharvest damage of azuki bean seeds, an important East Asian grain legume. The alpha-amylase in the midguts of these insects is inhibited by the alpha-amylase inhibitor (alpha AI) present in common bean seeds. Transformation of azuki bean with the alpha AI gene driven by the promoter of phytohemagglutinin results in high levels of alpha AI in the seeds and the complete block of bruchid development on the seeds. Zabrotes subfasciatus, a South and Central American bruchid that is storage pest of common bean, develops normally on the transgenic azuki bean.