Sensitization of renal transplant candidates by cryopreserved cadaveric venous or arterial allografts

Background. Cryopreserved cadaveric venous or arterial allografts are used in ESRD patients as an alternative to synthetic grafts for hemodialysis access. We evaluated the effect of these allografts on the PRA against HIA class I and II antigens in 11 ESRD patients awaiting a kidney transplant. Methods. Flow Cytometry using purified antigen coated beads (Flow PRA Beads(TM)) was used to determine PRA against HLA class I and II antigens. Results. Patients with no antibody prior to allograft implantation were all positive for HIA class I and II antibodies after implantation. Patients with anti-HLA class I and II antibodies prior to allografting, developed higher antibody titers. Of the 11 patients that received cryopreserved cadaveric allografts no deaths were reported. Two grafts were removed due to thrombosis consistent with rejection. Conclusion. Recipients of cadaveric venous or arterial allografts elicit an HLA antibody response attesting to the antigenicity of cryopreserved cadaveric allografts.