Feasibility of circulating miRNA microarray analysis from archival plasma samples

被引：22
作者：
Callari, Maurizio [1 ,2 ]
Tiberio, Paola [1 ]
De Cecco, Loris [2 ]
Cavadini, Elena [1 ]
Dugo, Matteo [2 ]
Ghimenti, Chiara [3 ]
Daidone, Maria Grazia [1 ]
Canevari, Silvana [2 ,4 ]
Appierto, Valentina [1 ]
机构：
[1] Fdn IRCCS Ist Nazl Tumori, Unit Biomarkers, Dept Expt Oncol & Mol Med, I-20133 Milan, Italy
[2] Fdn IRCCS Ist Nazl Tumori, Dept Expt Oncol & Mol Med, Funct Genom Core Facil, I-20133 Milan, Italy
[3] Edo & Elvo Tempia Fdn, Canc Genom Lab, Biella, Italy
[4] Fdn IRCCS Ist Nazl Tumori, Unit Mol Therapies, Dept Expt Oncol & Mol Med, I-20133 Milan, Italy
来源：
ANALYTICAL BIOCHEMISTRY | 2013年 / 437卷 / 02期
关键词：
miRNA; Plasma; Blood; Circulating miRNA; Agilent; Microarray; MICRORNAS; FENRETINIDE; CANCER; TRIAL; SERUM;
D O I：
10.1016/j.ab.2013.03.002
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
MicroRNAs have been found to be deregulated in several diseases and, due to their high stability in body fluids, represent promising noninvasively detectable biomarkers. However, numerous technical variables can affect accurate measurement of circulating miRNAs. Using a microarray-based method we assessed the: (i) adequate intra- and inter-array reproducibility of miRNA profiling; (ii) feasibility of using archival plasma samples stored for an extended period of time and available in limited amounts; (iii) good correlation between different batches; and (iv) time-dependent increase of background signals close to the chip expiration date. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码：123 / 125
页数：3
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