Shrink-induced sorting using integrated nanoscale magnetic traps

被引：23

作者：

Nawarathna, Dharmakeerthi ^{[1
]}

Norouzi, Nazila ^{[1
]}

McLane, Jolie ^{[1
]}

Sharma, Himanshu ^{[2
]}

Sharac, Nicholas ^{[3
]}

Grant, Ted ^{[4
]}

Chen, Aaron ^{[2
]}

Strayer, Scott ^{[1
]}

Ragan, Regina ^{[2
]}

Khine, Michelle ^{[1
,2
]}

机构：

[1] Univ Calif Irvine, Dept Biomed Engn, Irvine, CA 92697 USA

[2] Univ Calif Irvine, Dept Chem Engn, Irvine, CA 92697 USA

[3] Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA

[4] Univ Calif Irvine, Dept Phys & Astron, Irvine, CA 92697 USA

来源：

APPLIED PHYSICS LETTERS | 2013年 / 102卷 / 06期

基金：

美国国家科学基金会;

关键词：

CELLS; SEPARATION;

D O I：

10.1063/1.4790191

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We present a plastic microfluidic device with integrated nanoscale magnetic traps (NSMTs) that separates magnetic from non-magnetic beads with high purity and throughput, and unprecedented enrichments. Numerical simulations indicate significantly higher localized magnetic field gradients than previously reported. We demonstrated >20 000-fold enrichment for 0.001% magnetic bead mixtures. Since we achieve high purity at all flow-rates tested, this is a robust, rapid, portable, and simple solution to sort target species from small volumes amenable for point-of-care applications. We used the NSMT in a 96 well format to extract DNA from small sample volumes for quantitative polymerase chain reaction (qPCR). (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4790191]

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