Gut Microbiota Composition in Male Rat Models under Different Nutritional Status and Physical Activity and Its Association with Serum Leptin and Ghrelin Levels

被引：372

作者：

Isabel Queipo-Ortuno, Maria ^{[1
,2
]}

Maria Seoane, Luisa ^{[2
,3
]}

Murri, Mora ^{[1
]}

Pardo, Maria ^{[2
,3
]}

Miguel Gomez-Zumaquero, Juan ^{[4
]}

Cardona, Fernando ^{[1
,2
]}

Casanueva, Felipe ^{[2
,5
]}

Tinahones, Francisco J. ^{[2
,6
]}

机构：

[1] Virgen de la Victoria Hosp FIMABIS, Biomed Res Lab, Malaga, Spain

[2] Inst Salud Carlos III, CIBER Physiopathol Obes & Nutr CIBEROBN, Madrid, Spain

[3] Santiago de Compostela CHUS SERGAS, Hlth Res Inst Santiago de Compostela IDIS, Univ Hosp Complex Santiago de Compostela, Santiago De Compostela, Spain

[4] Civil Hosp FIMABIS, Mol Biol Lab, Malaga, Spain

[5] Clin Hosp Santiago de Compostela, Endocrinol & Nutr Serv, Santiago De Compostela, Spain

[6] Virgen de la Victoria Hospital, Endocrinol & Nutr Serv, Malaga, Spain

来源：

PLOS ONE | 2013年 / 8卷 / 05期

关键词：

ANOREXIA-NERVOSA; RIBOSOMAL-RNA; SELF-STARVATION; GROWTH-HORMONE; BACTERIA; OBESE; SECRETION; PRIMERS; POPULATION; METABOLISM;

D O I：

10.1371/journal.pone.0065465

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Backgrount: Several evidences indicate that gut microbiota is involved in the control of host energy metabolism. Objective: To evaluate the differences in the composition of gut microbiota in rat models under different nutritional status and physical activity and to identify their associations with serum leptin and ghrelin levels. Methods: In a case control study, forty male rats were randomly assigned to one of these four experimental groups: ABA group with food restriction and free access to exercise; control ABA group with food restriction and no access to exercise; exercise group with free access to exercise and feed ad libitum and ad libitum group without access to exercise and feed ad libitum. The fecal bacteria composition was investigated by PCR-denaturing gradient gel electrophoresis and real-time qPCR. Results: In restricted eaters, we have found a significant increase in the number of Proteobacteria, Bacteroides, Clostridium, Enterococcus, Prevotella and M. smithii and a significant decrease in the quantities of Actinobacteria, Firmicutes, Bacteroidetes, B. coccoides-E. rectale group, Lactobacillus and Bifidobacterium with respect to unrestricted eaters. Moreover, a significant increase in the number of Lactobacillus, Bifidobacterium and B. coccoides-E. rectale group was observed in exercise group with respect to the rest of groups. We also found a significant positive correlation between the quantity of Bifidobacterium and Lactobacillus and serum leptin levels, and a significant and negative correlation among the number of Clostridium, Bacteroides and Prevotella and serum leptin levels in all experimental groups. Furthermore, serum ghrelin levels were negatively correlated with the quantity of Bifidobacterium, Lactobacillus and B. coccoides-Eubacterium rectale group and positively correlated with the number of Bacteroides and Prevotella. Conclusions: Nutritional status and physical activity alter gut microbiota composition affecting the diversity and similarity. This study highlights the associations between gut microbiota and appetite-regulating hormones that may be important in terms of satiety and host metabolism.

引用

页数：11