Localization of the antigenic sites of newcastle disease virus nucleocapsid using a panel of monoclonal antibodies

被引：9

作者：

Ahmad-Raus, Raha ^{[2
]}

Ali, Abdul Manaf ^{[1
,3
]}

Tan, Wen Slang ^{[1
]}

Salleh, Hamzah Mohd ^{[2
]}

Eshaghi, Majid ^{[1
,4
]}

Yusoff, Khatijah ^{[1
]}

机构：

[1] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Microbiol, Serdang 43400, Selangor, Malaysia

[2] Int Islamic Univ Malaysia, Fac Engn, Kuala Lumpur 53100, Malaysia

[3] Univ Darul Iman Malaysia, Fac Agr & Biotechnol, Kuala Terengganu 21300, Terengganu, Malaysia

[4] Genome Inst Singapore, Genome, Singapore

来源：

RESEARCH IN VETERINARY SCIENCE | 2009年 / 86卷 / 01期

关键词：

Monoclonal antibody; Nucleocapsid; Antigenic sites; Newcastle disease virus; ESCHERICHIA-COLI; RABIES VIRUS; PROTEIN GENE; NUCLEOTIDE-SEQUENCE; N-PROTEIN; NUCLEOPROTEIN; TYPE-1; CHROMATOGRAPHY; DETERMINANTS; GLYCOPROTEIN;

D O I：

10.1016/j.rvsc.2008.05.013

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A panel of six monoclonal antibodies (mAbs) against the nucleocapsid (NP) protein of Newcastle disease virus (NDV) was produced by immunization of Balb/c mice with purified recombinant NP protein. Western Blot analysis showed that all the mAbs recognized linearized NP epitopes. Three different NP antigenic sites were identified using deleted truncated NP mutants purified from Escherichia coli. One of the antigenic sites was located at the C-terminal end (residues 441 to 489) of the NP protein. Two other antigenic sites were located within the N-terminal end (residues 26-121 and 122-375). This study demonstrates that the N- and C-terminal ends of the NP proteins are responsible in eliciting immune response, thus it is most likely that these ends are exposed on the NP. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：174 / 182

页数：9

共 50 条

[1] Antigenic diversity and similarities detected in avian paramyxovirus type 1 (Newcastle disease virus) isolates using monoclonal antibodies [J].