Natural phage nanoparticle-mediated real-time immuno-PCR for ultrasensitive detection of protein marker

被引：22

作者：

Zhang, Heng ^{[1
]}

Xu, Ye ^{[1
]}

Huang, Qiuying ^{[1
]}

Yi, Changqing ^{[2
]}

Xiao, Tan ^{[2
]}

Li, Qingge ^{[1
]}

机构：

[1] Xiamen Univ, Sch Life Sci, Dept Biomed Sci, Engn Res Ctr Mol Diagnost,Minist Educ, Xiamen 361005, Peoples R China

[2] Sun Yat Sen Univ, Sch Engn, Guangzhou 510275, Guangdong, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 36期

关键词：

ASSAY; REAGENTS; DISPLAY;

D O I：

10.1039/c3cc40688a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Current immuno-PCR methods either use bare or nanostructured DNA as a reporter or combine phage display for antigen binding and reporting, however, they are often complex to carry out and lack universality. We present a novel and universal design of immuno-PCR termed natural phage nanoparticle-mediated real-time immuno-PCR.

引用

页码：3778 / 3780

页数：3

共 17 条

[1] In-situ immun-PCR to detect antigens [J].

Cao, Y ;

Kopplow, K ;

Liu, GY .

LANCET, 2000, 356 (9234) :1002-1003

[2]

Fields R, 1972, Methods Enzymol, V25, P464, DOI 10.1016/S0076-6879(72)25042-X

[3]

Furuya D, 2001, CLIN CHEM, V47, P1475

[4] Phage display mediated immuno-PCR [J].

Guo, Yong-Chao ;

Zhou, Ya-Feng ;

Zhang, Xian-En ;

Zhang, Zhi-Ping ;

Qiao, Yan-Mei ;

Bi, Li-Jun ;

Wen, Ji-Kai ;

Liang, Mi-Fang ;

Zhang, Ji-Bin .

NUCLEIC ACIDS RESEARCH, 2006, 34 (08)

[5] Site-specific DNA-antibody conjugates for specific and sensitive immuno-PCR [J].

Kazane, Stephanie A. ;

Sok, Devin ;

Cho, Edward H. ;

Uson, Maria Loressa ;

Kuhn, Peter ;

Schultz, Peter G. ;

Smider, Vaughn V. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (10) :3731-3736

[6] Noncompetitive Phage Anti-Immunocomplex Real-Time Polymerase Chain Reaction for Sensitive Detection of Small Molecules [J].

Kim, Hee-Joo ;

McCoy, Mark ;

Gee, Shirley J. ;

Gonzalez-Sapienza, Gualberto G. ;

Hammock, Bruce D. .

ANALYTICAL CHEMISTRY, 2011, 83 (01) :246-253

[7] A new class of homogeneous nucleic acid probes based on specific displacement hybridization [J].

Li, QQ ;

Luan, GY ;

Guo, QP ;

Liang, JX .

NUCLEIC ACIDS RESEARCH, 2002, 30 (02) :5

[8] DEVELOPMENT OF A 2-SITE IMMUNO-PCR ASSAY FOR HEPATITIS-B SURFACE-ANTIGEN [J].

MAIA, M ;

TAKAHASHI, H ;

ADLER, K ;

GARLICK, RK ;

WANDS, JR .

JOURNAL OF VIROLOGICAL METHODS, 1995, 52 (03) :273-286

[9] A liposome-PCR assay for the ultrasensitive detection of biological toxins [J].

Mason, JT ;

Xu, LX ;

Sheng, ZM ;

O'Leary, TJ .

NATURE BIOTECHNOLOGY, 2006, 24 (05) :555-557

[10] Lmmuno-PCR: high sensitivity detection of proteins by nucleic acid amplification [J].

Niemeyer, CM ;

Adler, M ;

Wacker, R .

TRENDS IN BIOTECHNOLOGY, 2005, 23 (04) :208-216

← 1 2 →