Cross-protective efficacy of dendritic cells targeting conserved influenza virus antigen expressed by Lactobacillus plantarum

被引:30
作者
Yang, Wen-Tao [1 ]
Shi, Shao-Hua [1 ]
Yang, Gui-Lian [1 ]
Jiang, Yan-Long [1 ]
Zhao, Liang [1 ]
Li, Yu [1 ]
Wang, Chun-Feng [1 ]
机构
[1] Jilin Agr Univ, Coll Anim Sci & Technol, Jilin Prov Engn Res Ctr Anim Probiot, Engn Res Ctr Chinese Minist Educ Edible & Med Fun, Changchun 130118, Peoples R China
来源
SCIENTIFIC REPORTS | 2016年 / 6卷
基金
中国国家自然科学基金;
关键词
T-CELLS; IMMUNE-RESPONSES; VACCINE; INFECTION; MICE; H9N2; NUCLEOPROTEIN; IMMUNOPROTECTION; DIFFERENTIATION; HEMAGGLUTININ;
D O I
10.1038/srep39665
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Avian influenza virus (AIV) can infect birds and mammals, including humans, and are thus a serious threat to public health. Vaccination is vital for controlling AIV circulation. In this study, we generated a recombinant lactobacillus expressing the NP-M1-DCpep of H9N2 avian influenza virus and evaluated the activation effect of NC8-pSIP409-NP-M1-DCpep on dendritic cells (DCs) in a mouse model. The specific mucosal antibody responses and B and T cell responses in lymphoid tissues were also characterized. Importantly, we confirmed that specific CD8 T cells presented in vitro and antigen-specific cytotoxicity (activated the expression of CD107a) and in vivo antigen-specific cytotoxicity after vaccination. The adoptive transfer of NC8-pSIP409-NP-M1-DCpep-primed CD8(+) T cells into NOD-SCID mice resulted in effective protection against mouse-adapted AIV infection. In addition, we observed protection in immunized mice challenged with mouse-adapted H9N2 AIV and H1N1 influenza virus, as evidenced by reductions in the lung virus titers, improvements in lung pathology, and weight loss and complete survival. Our data are promising for the generation of effective, non-traditional influenza vaccines against AIVs.
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页数:20
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