Trmt61B is a methyltransferase responsible for 1-methyladenosine at position 58 of human mitochondrial tRNAs

被引：174

作者：

Chujo, Takeshi ^{[1
]}

Suzuki, Tsutomu ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan

来源：

RNA | 2012年 / 18卷 / 12期

关键词：

Trmt61B; 1-methyladenosine; tRNA; RNA modification; mitochondria; M(1)A58 METHYLTRANSFERASE; SACCHAROMYCES-CEREVISIAE; CRYSTAL-STRUCTURE; LACTIC-ACIDOSIS; CELL-GROWTH; IDENTIFICATION; PROTEINS; ENCEPHALOPATHY; COMPILATION; BIOGENESIS;

D O I：

10.1261/rna.035600.112

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In human mitochondria, 1-methyladenosine (m(1)A) occurs at position 58 of tRNA(Leu(UUR)). In addition, partial m(1)A58 modifications have been found in human mitochondrial tRNA(Lys) and tRNA(Ser(UCN)). We identified human Trmt61B, which encodes a mitochondria-specific tRNA methyltransferase responsible for m(1)A58 in these three tRNAs. Trmt61B is dominantly localized to the mitochondria. m(1)A58 formation in human mitochondrial tRNA(Leu(UUR)) could be reconstituted in vitro using recombinant Trmt61B in the presence of Ado-Met as a methyl donor. Unlike the cytoplasmic tRNA m(1)A58 methyltransferase that consists of an alpha 2 beta 2 heterotetramer formed by Trmt61A and Trmt6, Trmt61B formed a homo-oligomer (presumably a homotetramer) that resembled the bacterial homotetrameric m(1)A58 methyltransferase. The bacterial origin of Trmt61B is supported by the results of the phylogenetic analysis.

引用

页码：2269 / 2276

页数：8

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