High-Throughput Screening of Substrate Chemistry for Embryonic Stem Cell Attachment, Expansion, and Maintaining Pluripotency

被引：24
|
作者：
Zonca, Michael R., Jr. [1 ]
Yune, Philip S. [2 ,3 ]
Heldt, Caryn L. [2 ,3 ]
Belfort, Georges [2 ,3 ]
Xie, Yubing [1 ]
机构：
[1] SUNY Albany, Coll Nanoscale Sci & Engn, Albany, NY USA
[2] Rensselaer Polytech Inst, Howard P Isermann Dept Chem & Biol Engn, Troy, NY 12180 USA
[3] Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA
来源：
MACROMOLECULAR BIOSCIENCE | 2013年 / 13卷 / 02期
基金：
美国国家科学基金会;
关键词：
adhesion; high-throughput screening; monomer library; pluripotency; stem cells; TERM SELF-RENEWAL; DIFFERENTIATION; CULTURE; SURFACES; GROWTH; BIOMATERIALS; FIBRONECTIN; ADSORPTION; BINDING; SYSTEMS;
D O I：
10.1002/mabi.201200315
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Appropriate surface attachment is essential for growing embryonic stem (ES) cells in an undifferentiated state. It is challenging to identify the optimal surface chemistry of the substrata for ES cell attachment and maintenance. Using a rapid, high-throughput polymerization and screening platform with a comprehensive library of 66 monomer-grafted membrane surfaces, the optimal substrate, N-[3-(dimethylamino)propyl] methacrylamide (DMAPMA) has been identified to support strong attachment, high expansion capacity, and long-term self-renewal of ES cells (up to 7 passages). This monomer-based, chemically defined, scalable, sustainable, relatively inexpensive, covalently grafted, and controllable polymeric substrate provides a new opportunity to manipulate surface chemistry for pluripotent stem culture.
引用
收藏
页码：177 / 190
页数：14
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