Molecular identification of Tomato spotted wilt virus on pepper and tobacco in Republic of Srpska (Bosnia and Herzegovina)

被引：3
|
作者：
Delic, Duska [1 ]
Balech, Bachir [2 ]
Radulovic, Mariana [1 ]
Duric, Zorica [1 ]
Lolic, Biljana [1 ]
Santamaria, Monica [2 ]
Duric, Gordana [1 ]
机构：
[1] Univ Banja Luka, Fac Agr, Bulevar Vojvode Petra Bojovica 1A, Banja Luka 78000, Bosnia & Herceg
[2] CNR, Inst Biomembranes Bioenerget & Mol Biotechnol, Via Amendola 165-A, I-70126 Bari, Italy
来源：
EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2018年 / 150卷 / 03期
关键词：
TSWV; Capsicum annuum; Nicotiana tabacum; DAS ELISA; PCR; Sequencing; Phylogeny; 1ST REPORT; SERBIA; TOSPOVIRUSES; MONTENEGRO; POLYMERASE; RESISTANCE; CROPS;
D O I：
10.1007/s10658-017-1313-7
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Surveys for TSWV presence and distribution on vegetable, ornamental and tobacco plants were conducted during 2016 in open fields and greenhouses in different locations in Republic of Srpska, district of Bosnia and Herzegovina. One hundred and twelve samples were taken and preliminarily tested for TSWV presence with DAS-ELISA. Positive samples were double-checked in RT-PCR assays using specific primer sets and subsequently partial sequences of RdRp and N region genes were characterized. Serological and molecular results revealed twenty-seven TSWV positive samples (two peppers and twenty-five tobacco plants). Moreover, phylogenetic analyses showed that these isolates share the same gene pool and a similar evolutionary pattern mainly with other isolates present in Europe and Russia.
引用
收藏
页码：785 / 789
页数：5
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