Synthesis and enzymatic incorporation of morpholino thymidine-5′-triphosphate in DNA fragments

被引：17

作者：

Marciacq, F

Sauvaigo, S

Issartel, JP

Mouret, JF

Molko, D

机构：

[1] CEA, Dept Rech Fondamentale Mat Condensee, Serv Chim Inorgan & Biol, Lab Les Acides Nucl, F-38054 Grenoble 9, France

[2] CEA, Dept Biol Mol & Struct, Lab Bioenerget Cellulaire & Pathol, F-38054 Grenoble, France

[3] CEA, Soc Genome Express SA, F-38054 Grenoble 9, France

来源：

TETRAHEDRON LETTERS | 1999年 / 40卷 / 25期

关键词：

D O I：

10.1016/S0040-4039(99)00842-4

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

4-(carboxymethyl)-2-(thymidin-9-yl)-6-(hydroxymethyl)morpholine-6-triphosphate (morpholino thymidine-5'-triphosphate) was synthesised from 1-(beta-D-erythro-pentofuranosyl) thymine. It was fully characterised by NMR, UV and mass spectrometry. Taq polymerase enzymatic incorporation of this nucleotide analogue into DNA fragments was investigated. Morpholino thymidine-5'-triphosphate was incorporated in a base-specific process and acted as a novel chain terminator in DNA sequencing, similarly to the corresponding dideoxynucleotide. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：4673 / 4676

页数：4

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