Synthesis and enzymatic incorporation of morpholino thymidine-5′-triphosphate in DNA fragments

被引：17
|
作者：
Marciacq, F
Sauvaigo, S
Issartel, JP
Mouret, JF
Molko, D
机构：
[1] CEA, Dept Rech Fondamentale Mat Condensee, Serv Chim Inorgan & Biol, Lab Les Acides Nucl, F-38054 Grenoble 9, France
[2] CEA, Dept Biol Mol & Struct, Lab Bioenerget Cellulaire & Pathol, F-38054 Grenoble, France
[3] CEA, Soc Genome Express SA, F-38054 Grenoble 9, France
来源：
TETRAHEDRON LETTERS | 1999年 / 40卷 / 25期
关键词：
D O I：
10.1016/S0040-4039(99)00842-4
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
4-(carboxymethyl)-2-(thymidin-9-yl)-6-(hydroxymethyl)morpholine-6-triphosphate (morpholino thymidine-5'-triphosphate) was synthesised from 1-(beta-D-erythro-pentofuranosyl) thymine. It was fully characterised by NMR, UV and mass spectrometry. Taq polymerase enzymatic incorporation of this nucleotide analogue into DNA fragments was investigated. Morpholino thymidine-5'-triphosphate was incorporated in a base-specific process and acted as a novel chain terminator in DNA sequencing, similarly to the corresponding dideoxynucleotide. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：4673 / 4676
页数：4
相关论文
共 50 条
12345