Visualization and exploration of Tcf/Lef function using a highly responsive Wnt/β-catenin signaling-reporter transgenic zebrafish

被引：97

作者：

Shimizu, Nobuyuki ^{[1
]}

Kawakami, Koichi ^{[2
]}

Ishitani, Tohru ^{[1
]}

机构：

[1] Kyushu Univ, Div Cell Regulat Syst, Dept Immunobiol & Neurosci, Med Inst Bioregulat,Higashi Ku, Fukuoka 8128582, Japan

[2] Natl Inst Genet, Div Mol & Dev Biol, Mishima, Shizuoka 4118540, Japan

来源：

DEVELOPMENTAL BIOLOGY | 2012年 / 370卷 / 01期

关键词：

Tcf/Lef; Wnt/beta-catenin signaling; Zebrafish; MIDBRAIN-HINDBRAIN BOUNDARY; LATERAL-LINE DEVELOPMENT; STEM-CELLS; IN-VIVO; POSTEMBRYONIC DEVELOPMENT; TRANSCRIPTION FACTOR; WNT; LEF1; DIFFERENTIATION; REGENERATION;

D O I：

10.1016/j.ydbio.2012.07.016

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Evolutionarily conserved Tcf/Lef transcription factors (Lef1, Tcf7, Tcf711, and Tcf712) mediate gene expression regulated by Wnt/beta-catenin signaling, which has multiple roles in early embryogenesis, organogenesis, adult tissue homeostasis, and tissue regeneration. However, the spatiotemporal dynamics of Tcf/Lef activity during these events remain poorly understood. We generated stable transgenic zebrafish lines carrying a new Wnt/beta-catenin signaling reporter, Tcf/Lef-miniP:dGFP. The reporter revealed the transcriptional activities of four Tcf/Lef members controlled by Wnt/beta-catenin signaling, which were expressed in known Wnt/beta-catenin signaling-active sites during embryogenesis, organ development and growth, and tissue regeneration. We used the transgenic lines to demonstrate the contribution of Tcf/Lef-mediated Wnt/beta-catenin signaling to the development of the anterior lateral line, dorsal and secondary posterior lateral lines, and gill filaments. Thus, these reporter lines are highly useful tools for studying Tcf/Lef-mediated Wnt/beta-catenin signaling-dependent processes. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：71 / 85

页数：15