Three-dimensional evaluation of the spinal local neural network revealed by the high-voltage electron microscopy: a double immunohistochemical study

被引:11
作者
Oti, Takumi [1 ]
Satoh, Keita [1 ]
Saito, Kazuhiro [1 ]
Murata, Kazuyoshi [2 ]
Kawata, Mitsuhiro [3 ]
Sakamoto, Tatsuya [1 ]
Sakamoto, Hirotaka [1 ]
机构
[1] Okayama Univ, Ushimado Marine Inst, Grad Sch Nat Sci & Technol, Okayama 7014303, Japan
[2] Natl Inst Physiol Sci, Sect Brain Struct Informat, Support Ctr Brain Res, Okazaki, Aichi 4448585, Japan
[3] Kyoto Prefectural Univ Med, Dept Anat & Neurobiol, Kamigyo Ku, Kyoto 6028566, Japan
关键词
Three-dimensional analysis; Tomography; High-voltage electron microscopy; Ultrastructure; Neuroanatomy; Histological methodology; SACRAL PARASYMPATHETIC NUCLEUS; BRAIN; CORD; VISUALIZATION; SYSTEM;
D O I
10.1007/s00418-012-0976-6
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Three-dimensional (3-D) analysis of anatomical ultrastructures is important in biological research. However, 3-D image analysis on exact serial sets of ultra-thin sections from biological specimens is very difficult to achieve, and limited information can be obtained by 3-D reconstruction from these sections due to the small area that can be reconstructed. On the other hand, the high-penetration power of electrons by an ultra-high accelerating voltage enables thick sections of biological specimens to be examined. High-voltage electron microscopy (HVEM) is particularly useful for 3-D analysis of the central nervous system because considerably thick sections can be observed at the ultrastructure level. Here, we applied HVEM tomography assisted by light microscopy to a study of the 3-D chemical neuroanatomy of the rat lower spinal cord annotated by double-labeling immunohistochemistry. This powerful methodology is useful for studying molecular and/or chemical neuroanatomy at the 3-D ultrastructural level.
引用
收藏
页码:693 / 697
页数:5
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