Evaluation of the Effects of Homologous Platelet Gel on Healing Lower Extremity Wounds in Patients With Diabetes

被引:43
作者
Shan, Gui-Qiu [1 ,2 ]
Zhang, Ya-Ni [2 ]
Ma, Jing [2 ]
Li, Yan-Hui [2 ]
Zuo, Da-Ming [1 ]
Qiu, Jin-lang [2 ]
Cheng, Biao [2 ,3 ]
Chen, Zheng-Liang [1 ]
机构
[1] Southern Med Univ, Dept Immunol, Guangzhou 510515, Guangdong, Peoples R China
[2] Guangzhou Gen Hosp Guangzhou Mil Command, Guangzhou, Guangdong, Peoples R China
[3] PLA, Key Lab Trauma Treatment & Tissue Repair Trop Are, Guangzhou, Guangdong, Peoples R China
关键词
diabetic lower extremity wounds; homologous; platelet-rich plasma; proliferation; migration; NEUROPATHIC FOOT ULCERS; GROWTH-FACTORS; BECAPLERMIN; AMPUTATIONS;
D O I
10.1177/1534734613477113
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
The treatment of chronic diabetic wounds remains complicated, despite new insight into the cellular and molecular basis of wound healing and cutaneous regeneration. A growing body of clinical trials has shown that platelet release has a notable effectiveness on refractory ulcer healing. However, patients with chronic diabetic ulcers usually have poor general health, and the large-volume blood absence required to produce autologous platelet-rich plasma often causes adverse effects. To overcome the limitation, the homologous platelet gel (PG) from healthy donor was used for the treatment of chronic diabetic lower extremity wound in the study. We show here that homologous derived platelets significantly enhanced EVC304 cell and HaCaT cell proliferation and homologous PG was capable of prompting cell migration. Twenty-one patients with refractory diabetic lower extremity ulcers, who had no response to conventional treatments, were treated in this study. Our data indicated that homologous PG was effective for the enhancement and acceleration of diabetic lower extremity wounds healing. We propose that homologous PG appeared to enhance vascularization and epithelialization, which might induce a quicker healing process and and encourage controlled studies in future.
引用
收藏
页码:22 / 29
页数:8
相关论文
共 30 条
  • [11] E2F8 Contributes to Human Hepatocellular Carcinoma via Regulating Cell Proliferation
    Deng, Qing
    Wang, Qun
    Zong, Wei-Ying
    Zheng, Da-Li
    Wen, Yi-Xin
    Wang, Ke-Sheng
    Teng, Xiao-Mei
    Zhang, Xin
    Huang, Jian
    Han, Ze-Guang
    [J]. CANCER RESEARCH, 2010, 70 (02) : 782 - 791
  • [12] Driver VR, 2006, OSTOMY WOUND MANAG, V52, P68
  • [13] Wound healing and its impairment in the diabetic foot
    Falanga, V
    [J]. LANCET, 2005, 366 (9498) : 1736 - 1743
  • [14] Fang RC, 2008, BIOL-TARGETS THER, V2, P1
  • [15] Fivenson David P., 1997, Wound Repair and Regeneration, V5, P310, DOI 10.1046/j.1524-475X.1997.50405.x
  • [16] Frei R, 2008, ACTA CHIR ORTHOP TR, V75, P247
  • [17] Identification of an optimal concentration of platelet gel for promoting angiogenesis in human endothelial cells
    Giusti, Ilaria
    Rughetti, Anna
    D'Ascenzo, Sandra
    Millimaggi, Danilo
    Pavan, Antonio
    Dell'Orso, Luigi
    Dolo, Vincenza
    [J]. TRANSFUSION, 2009, 49 (04) : 771 - 778
  • [18] Hunt D, 2009, AM FAM PHYSICIAN, V80, P789
  • [19] Treatment of Diabetic Foot Ulcers Using a Blood Bank Platelet Concentrate
    Jeong, Seong-Ho
    Han, Seung-Kyu
    Kim, Woo-Kyung
    [J]. PLASTIC AND RECONSTRUCTIVE SURGERY, 2010, 125 (03) : 944 - 952
  • [20] Lacci Kathleen M., 2010, Yale Journal of Biology and Medicine, V83, P1