Acoustic focusing with engineered node locations for high-performance microfluidic particle separation

被引:35
作者
Fong, Erika J. [1 ,2 ]
Johnston, Amanda C. [1 ]
Notton, Timothy [3 ,6 ]
Jung, Seung-Yong [3 ,5 ]
Rose, Klint A. [1 ]
Weinberger, Leor S. [3 ,4 ,5 ]
Shusteff, Maxim [1 ]
机构
[1] Lawrence Livermore Natl Lab, Livermore, CA 94550 USA
[2] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA
[3] Gladstone Inst, Dept Virol & Immunol, San Francisco, CA 94158 USA
[4] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA
[5] Univ Calif San Francisco, Calif Inst Quantitat Biol QB3, San Francisco, CA 94158 USA
[6] Univ Calif San Francisco, Joint Grad Grp Bioengn, San Francisco, CA 94158 USA
关键词
ULTRASOUND STANDING-WAVE; SUSPENDED PARTICLES; FLOW; CELLS; ACOUSTOPHORESIS; SURFACE; MANIPULATION; DRIVEN; CHIP; SIZE;
D O I
10.1039/c4an00034j
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Acoustofluidic devices for manipulating microparticles in fluids are appealing for biological sample processing due to their gentle and high-speed capability of sorting cell-scale objects. Such devices are generally limited to moving particles toward locations at integer fractions of the fluid channel width (1/2, 1/4, 1/6, etc.). In this work, we introduce a unique approach to acoustophoretic device design that overcomes this constraint, allowing us to design the particle focusing location anywhere within the microchannel. This is achieved by fabricating a second fluid channel in parallel with the sample channel, separated from it by a thin silicon wall. The fluids in both channels participate to create the ultrasound resonance, while only one channel processes the sample, thus de-coupling the fluidic and acoustic boundaries. The wall placement and the relative widths of the adjacent channels define the particle focusing location. We investigate the operating characteristics of a range of these devices to determine the configurations that enable effective particle focusing and separation. The results show that a sufficiently thin wall negligibly affects focusing efficiency and location compared to a single channel without a wall, validating the success of this design approach without compromising separation performance. Using these principles to design and fabricate an optimized device configuration, we demonstrate high-efficiency focusing of microspheres, as well as separation of cell-free viruses from mammalian cells. These "transparent wall" acoustic devices are capable of over 90% extraction efficiency with 10 mm microspheres at 450 mu L min(-1), and of separating cells (98% purity), from viral particles (70% purity) at 100 mu L min(-1).
引用
收藏
页码:1192 / 1200
页数:9
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