共 56 条
Acoustic focusing with engineered node locations for high-performance microfluidic particle separation
被引:35
作者:
Fong, Erika J.
[1
,2
]
Johnston, Amanda C.
[1
]
Notton, Timothy
[3
,6
]
Jung, Seung-Yong
[3
,5
]
Rose, Klint A.
[1
]
Weinberger, Leor S.
[3
,4
,5
]
Shusteff, Maxim
[1
]
机构:
[1] Lawrence Livermore Natl Lab, Livermore, CA 94550 USA
[2] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA
[3] Gladstone Inst, Dept Virol & Immunol, San Francisco, CA 94158 USA
[4] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA
[5] Univ Calif San Francisco, Calif Inst Quantitat Biol QB3, San Francisco, CA 94158 USA
[6] Univ Calif San Francisco, Joint Grad Grp Bioengn, San Francisco, CA 94158 USA
来源:
关键词:
ULTRASOUND STANDING-WAVE;
SUSPENDED PARTICLES;
FLOW;
CELLS;
ACOUSTOPHORESIS;
SURFACE;
MANIPULATION;
DRIVEN;
CHIP;
SIZE;
D O I:
10.1039/c4an00034j
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
Acoustofluidic devices for manipulating microparticles in fluids are appealing for biological sample processing due to their gentle and high-speed capability of sorting cell-scale objects. Such devices are generally limited to moving particles toward locations at integer fractions of the fluid channel width (1/2, 1/4, 1/6, etc.). In this work, we introduce a unique approach to acoustophoretic device design that overcomes this constraint, allowing us to design the particle focusing location anywhere within the microchannel. This is achieved by fabricating a second fluid channel in parallel with the sample channel, separated from it by a thin silicon wall. The fluids in both channels participate to create the ultrasound resonance, while only one channel processes the sample, thus de-coupling the fluidic and acoustic boundaries. The wall placement and the relative widths of the adjacent channels define the particle focusing location. We investigate the operating characteristics of a range of these devices to determine the configurations that enable effective particle focusing and separation. The results show that a sufficiently thin wall negligibly affects focusing efficiency and location compared to a single channel without a wall, validating the success of this design approach without compromising separation performance. Using these principles to design and fabricate an optimized device configuration, we demonstrate high-efficiency focusing of microspheres, as well as separation of cell-free viruses from mammalian cells. These "transparent wall" acoustic devices are capable of over 90% extraction efficiency with 10 mm microspheres at 450 mu L min(-1), and of separating cells (98% purity), from viral particles (70% purity) at 100 mu L min(-1).
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页码:1192 / 1200
页数:9
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