Structure and properties of a (1→3)-β-D-glucan from ultrasound-degraded exopolysaccharides of a medicinal fungus

被引：39

作者：

Chen, Xia ^{[1
]}

Siu, Ka-Chai ^{[1
]}

Cheung, Yi-Ching ^{[1
]}

Wu, Jian-Yong ^{[1
]}

机构：

[1] Hong Kong Polytech Univ, State Key Lab Chinese Med & Mol Pharmacol Shenzhe, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2014年 / 106卷

关键词：

Cordyceps sinensis; Mycelial fermentation; Exopolysaccharide; Ultrasonic degradation; (1 -> 3)-beta-D-glucan; Moisture absorption; MYCELIAL FERMENTATION; ANTIOXIDANT ACTIVITY; MOISTURE-ABSORPTION; CHAIN CONFORMATION; POLYSACCHARIDES; DEACETYLATION; CULTURE; GLUCAN;

D O I：

10.1016/j.carbpol.2014.02.040

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A high molecular weight (MW) exopolysaccaride (EPS) fraction EPS1 was isolated from the fermentation broth of a medicinal fungus Cordyceps sinensis Cs-HK1 and partially degraded by high-intensity ultrasound (US) into a lower MW fraction EPS1U. EPS1U exhibited a single, symmetric peak on size exclusion chromatography with an average MW of 730 kDa by light scattering analysis. It had a much lower intrinsic viscosity (1.7 versus 15.6 dL/g) but a much higher solubility in water (77.5 versus 5.1 g/L) than EPS1U Based on methylation analysis and NMR spectrometry, the structure of EPS1U was deduced as a (1 -> 3)-beta-D-glucan with glucose side chains attached to 0-6 position at the branching points. EPS1U showed a high moisture absorption capability comparable to chitosan and urea, suggesting its potential as a moisturizing agent for food and cosmeceutical application. This is the first report on a high MW (1 -> 3)-beta-D-glucan isolated from EPS produced by Cordyceps sinensis. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：270 / 275

页数：6

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