Structure and properties of a (1→3)-β-D-glucan from ultrasound-degraded exopolysaccharides of a medicinal fungus

被引：37
|
作者：
Chen, Xia [1 ]
Siu, Ka-Chai [1 ]
Cheung, Yi-Ching [1 ]
Wu, Jian-Yong [1 ]
机构：
[1] Hong Kong Polytech Univ, State Key Lab Chinese Med & Mol Pharmacol Shenzhe, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2014年 / 106卷
关键词：
Cordyceps sinensis; Mycelial fermentation; Exopolysaccharide; Ultrasonic degradation; (1 -> 3)-beta-D-glucan; Moisture absorption; MYCELIAL FERMENTATION; ANTIOXIDANT ACTIVITY; MOISTURE-ABSORPTION; CHAIN CONFORMATION; POLYSACCHARIDES; DEACETYLATION; CULTURE; GLUCAN;
D O I：
10.1016/j.carbpol.2014.02.040
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A high molecular weight (MW) exopolysaccaride (EPS) fraction EPS1 was isolated from the fermentation broth of a medicinal fungus Cordyceps sinensis Cs-HK1 and partially degraded by high-intensity ultrasound (US) into a lower MW fraction EPS1U. EPS1U exhibited a single, symmetric peak on size exclusion chromatography with an average MW of 730 kDa by light scattering analysis. It had a much lower intrinsic viscosity (1.7 versus 15.6 dL/g) but a much higher solubility in water (77.5 versus 5.1 g/L) than EPS1U Based on methylation analysis and NMR spectrometry, the structure of EPS1U was deduced as a (1 -> 3)-beta-D-glucan with glucose side chains attached to 0-6 position at the branching points. EPS1U showed a high moisture absorption capability comparable to chitosan and urea, suggesting its potential as a moisturizing agent for food and cosmeceutical application. This is the first report on a high MW (1 -> 3)-beta-D-glucan isolated from EPS produced by Cordyceps sinensis. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：270 / 275
页数：6
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