microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells

被引：62

作者：

Mao, Yeqing ^{[1
]}

Chen, Hong ^{[1
]}

Lin, Yiwei ^{[1
]}

Xu, Xin ^{[1
]}

Hu, Zhenghui ^{[1
]}

Zhu, Yi ^{[1
]}

Wu, Jian ^{[1
]}

Xu, Xianglai ^{[1
]}

Zheng, Xiangyi ^{[1
]}

Xie, Liping ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Dept Urol, Affiliated Hosp 1, Hangzhou 310003, Zhejiang, Peoples R China

来源：

ONCOLOGY REPORTS | 2013年 / 30卷 / 01期

关键词：

microRNA-330; prostate cancer; migration; invasion; specificity protein 1; ENDOTHELIAL GROWTH-FACTOR; BOX-BINDING PROTEIN; TRANSCRIPTION FACTORS; EXPRESSION; SUPPRESSION; ACTIVATORS; THERAPY; GENES; LINES; RNA;

D O I：

10.3892/or.2013.2452

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

microRNAs (miRNAs), small non-coding RNAs, have emerged as key regulators of a large number of genes. The present study aimed to explore novel biological functions of miR-330 in the human prostate cancer cell lines DU145 and PC3. We confirmed that miR-330 was downregulated and inversely correlated with specificity protein 1 (Sp1) expression. Overexpression of miR-330 by transfection of a chemically synthesized miR-330 mimic induced a reduction in expression levels of the Sp1 protein, accompanied by significant suppression of cellular migration and invasion capability. In addition, the Sp1-knockdown experiments presented similar phenomena. Finally, the luciferase reporter assay validated Sp1 as the direct target of miR-330. These findings indicate that miR-330 acts as an anti-metastatic miRNA in prostate cancer.

引用

页码：327 / 333

页数：7

共 29 条

[1] The regulation of genes and genomes by small RNAs
Ambros, Victor
Chen, Xuemei
[J]. DEVELOPMENT, 2007, 134 (09): : 1635 - 1641
[2] [Anonymous], BR J CANC
[3] miRNA control of tumor cell invasion and metastasis
Baranwal, Somesh
Alahari, Suresh K.
[J]. INTERNATIONAL JOURNAL OF CANCER, 2010, 126 (06) : 1283 - 1290
[4] ASSEMBLY OF RECOMBINANT TFIID REVEALS DIFFERENTIAL COACTIVATOR REQUIREMENTS FOR DISTINCT TRANSCRIPTIONAL ACTIVATORS
CHEN, JL
ATTARDI, LD
VERRIJZER, CP
YOKOMORI, K
TJIAN, R
[J]. CELL, 1994, 79 (01) : 93 - 105
[5] Increased expression of AP2 and Sp1 transcription factors in human thyroid tumors: a role in NIS expression regulation?
Chiefari, E
Brunetti, A
Arturi, F
Bidart, JM
Russo, D
Schlumberger, M
Filetti, S
[J]. BMC CANCER, 2002, 2 (1)
[6] Betulinic acid inhibits prostate cancer growth through inhibition of specificity protein transcription factors
Chintharlapalli, Sudhakar
Papineni, Sabitha
Ramaiah, Shashi K.
Safe, Stephen
[J]. CANCER RESEARCH, 2007, 67 (06) : 2816 - 2823
[7] Assembly of partial TFIID complexes in mammalian cells reveals distinct activities associated with individual TATA box-binding protein-associated factors
Furukawa, T
Tanese, N
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (38) : 29847 - 29856
[8] Characterization of microRNA expression levels and their biological correlates in human cancer cell lines
Gaur, Arti
Jewell, David A.
Liang, Yu
Ridzon, Dana
Moore, Jason H.
Chen, Caifu
Ambros, Victor R.
Israel, Mark A.
[J]. CANCER RESEARCH, 2007, 67 (06) : 2456 - 2468
[9] A microRNA in a multiple-turnover RNAi enzyme complex
Hutvágner, G
Zamore, PD
[J]. SCIENCE, 2002, 297 (5589) : 2056 - 2060
[10] MicroRNAs in Cancer: Small Molecules With a Huge Impact
Iorio, Marilena V.
Croce, Carlo M.
[J]. JOURNAL OF CLINICAL ONCOLOGY, 2009, 27 (34) : 5848 - 5856

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