microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells

被引：63

作者：

Mao, Yeqing ^{[1
]}

Chen, Hong ^{[1
]}

Lin, Yiwei ^{[1
]}

Xu, Xin ^{[1
]}

Hu, Zhenghui ^{[1
]}

Zhu, Yi ^{[1
]}

Wu, Jian ^{[1
]}

Xu, Xianglai ^{[1
]}

Zheng, Xiangyi ^{[1
]}

Xie, Liping ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Dept Urol, Affiliated Hosp 1, Hangzhou 310003, Zhejiang, Peoples R China

来源：

ONCOLOGY REPORTS | 2013年 / 30卷 / 01期

关键词：

microRNA-330; prostate cancer; migration; invasion; specificity protein 1; ENDOTHELIAL GROWTH-FACTOR; BOX-BINDING PROTEIN; TRANSCRIPTION FACTORS; EXPRESSION; SUPPRESSION; ACTIVATORS; THERAPY; GENES; LINES; RNA;

D O I：

10.3892/or.2013.2452

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

microRNAs (miRNAs), small non-coding RNAs, have emerged as key regulators of a large number of genes. The present study aimed to explore novel biological functions of miR-330 in the human prostate cancer cell lines DU145 and PC3. We confirmed that miR-330 was downregulated and inversely correlated with specificity protein 1 (Sp1) expression. Overexpression of miR-330 by transfection of a chemically synthesized miR-330 mimic induced a reduction in expression levels of the Sp1 protein, accompanied by significant suppression of cellular migration and invasion capability. In addition, the Sp1-knockdown experiments presented similar phenomena. Finally, the luciferase reporter assay validated Sp1 as the direct target of miR-330. These findings indicate that miR-330 acts as an anti-metastatic miRNA in prostate cancer.

引用

页码：327 / 333

页数：7

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