Porous star-star polyelectrolyte multilayers for protein binding

被引：9

作者：

Liu, Weijing ^{[1
]}

Wijeratne, Salinda ^{[2
]}

Yang, Liu ^{[1
]}

Bruening, Merlin ^{[1
,3
]}

机构：

[1] Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA

[2] Michigan State Univ, Dept Chem, E Lansing, MI 48824 USA

[3] Univ Notre Dame, Dept Chem & Biomol Engn, Notre Dame, IN 46556 USA

来源：

POLYMER | 2018年 / 138卷

基金：

美国国家科学基金会;

关键词：

Star-polyelectrolyte; Layer-by-layer; Porosity; Protein binding; Membrane; POLY(ACRYLIC ACID)-CONTAINING FILMS; TRANSFER RADICAL POLYMERIZATION; MRI CONTRAST AGENTS; THIN-FILMS; MEMBRANE ADSORBERS; BLOCK-COPOLYMERS; IONIC-STRENGTH; DRUG-DELIVERY; GENE DELIVERY; LOW PH;

D O I：

10.1016/j.polymer.2018.01.055

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

This work employs layer-by-layer adsorption of star-poly[2-(dimethylamino) ethyl methacrylate] (star-PDMAEMA) and star-poly(acrylic acid) (star-PAA) to create porous coatings that bind the equivalent of many monolayers of lysozyme. AFM images of (star-PDMAEMA/star-PAA)(n) films on gold-coated wafers reveal pore diameters ranging from 100-550 nm, depending on the number of arms in the polymer and the number of bilayers, n, in the films. (Star-PDMAEMA/star-PAA) n coatings on Au-coated wafers bind the equivalent of up to 150 nm of lysozyme, and binding is greater for films containing star-PAA with 3, rather than 4, arms. Deposition of star-PAA/star-PDMAEMA films in porous hydroxylated nylon gives membranes that bind proteins via ion-exchange interactions. Such membranes capture as much as 120mg of lysozyme per cm(3) of membrane (equilibrium binding capacity), which is more than twice the capacity of commercial ion-exchange membranes, but films are unstable at neutral and high pH. (C) 2018 Elsevier Ltd. All rights reserved.

引用

页码：267 / 274

页数：8

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