Combined Antibacterial Effect of Sodium Hypochlorite and Root Canal Sealers against Enterococcus faecalis Biofilms in Dentin Canals

被引:43
作者
Du, Tianfeng [1 ,2 ]
Wang, Zhejun [2 ,3 ,4 ]
Shen, Ya [2 ,5 ]
Ma, Jingzhi [6 ]
Cao, Yingguang [6 ]
Haapasalo, Markus [2 ]
机构
[1] Zhengzhou Univ, Dept Stomatol, Affiliated Hosp 1, Zhengzhou 450052, Peoples R China
[2] Univ British Columbia, Dept Oral Biol & Med Sci, Div Endodont, Fac Dent, Vancouver, BC V5Z 1M9, Canada
[3] Wuhan Univ, State Key Lab Breeding Base Basic Sci Stomatol Hu, Wuhan 430072, Peoples R China
[4] Wuhan Univ, Key Lab Oral Biomed, Minist Educ, Sch & Hosp Stomatol, Wuhan 430072, Peoples R China
[5] Univ British Columbia, Dept Mat Engn, Vancouver, BC V5Z 1M9, Canada
[6] Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Stomatol, Wuhan 430074, Peoples R China
基金
中国国家自然科学基金; 加拿大创新基金会;
关键词
Confocal laser scanning microscopy; dentin; Enterococcus faecalis; sealer; sodium hypochlorite; ENDODONTIC DISINFECTING SOLUTIONS; LASER-SCANNING MICROSCOPY; BACTERIA; TUBULES; VIABILITY; INFECTION; SURVIVAL; EFFICACY; CULTURE; INVITRO;
D O I
10.1016/j.joen.2015.04.023
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Introduction: The present study aimed to evaluate the antibacterial effect of the combined use of sodium hypochlorite (NaOCl) and root canal sealers on Enterococcus faecalis biofilms using a dentin infection model. Methods: Cells of E. faecalis were introduced into the dentinal tubules by centrifugation and incubated in brain-heart infusion for 3 weeks. The biofilms in dentin were first subjected to 5% NaOCl or sterile water for 10 minutes followed by an equal thickness of AH Plus (Dentsply International Inc, York, PA), Endosequence BC Sealer (Brasseler USA, Savannah, GA), or MTA Fillapex (Angelus Industria de Produtos Odontologicos S/A, Londrina, Brazil) placed on the root canal wall of the dentin specimens for 7, 30, and 60 days. Gutta-percha and water were used in a similar manner as controls. The proportions of dead and live bacteria inside the dentinal tubules were assessed by confocal laser scanning microscopy and viability staining. Results: The combined use of NaOCl and sealers (30 and 60 days) killed significantly more bacteria than NaOCl or sealers alone (P < .05). NaOCl + MTA Fillapex was the most effective antibacterial combination by killing 83% bacteria in dentin tubules in 60 days. Thirty and 60 days of exposure to the sealers resulted in significantly more dead bacteria in dentin biofilms than 7-day exposures (P < .05). Conclusions: The placement of root canal sealer after NaOCl treatment enhanced antibacterial effects against E. faecalis in the dentinal tubules. Little additional effect was obtained after 30 days of exposure to sealers.
引用
收藏
页码:1294 / 1298
页数:5
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