Activity of the Bacillus thuringiensisNprRNprX cellcell communication system is co-ordinated to the physiological stage through a complex transcriptional regulation

被引：27

作者：

Dubois, Thomas ^{[1
,2
]}

Perchat, Stephane ^{[1
,2
]}

Verplaetse, Emilie ^{[1
,2
]}

Gominet, Myriam ^{[3
]}

Lemy, Christelle ^{[1
,2
]}

Aumont-Nicaise, Magali ^{[4
]}

Grenha, Rosa ^{[5
]}

Nessler, Sylvie ^{[4
,5
]}

Lereclus, Didier ^{[1
,2
]}

机构：

[1] INRA, Micalis UMR1319, F-78280 La Miniere, Guyancourt, France

[2] AgroParisTech, Micalis UMR1319, F-78350 Jouy En Josas, France

[3] Inst Pasteur, CNRS URA2172, Unite Biol Bacteries Pathogenes Gram Positif, F-75015 Paris, France

[4] Univ Paris 11, CNRS UMR8619, Inst Biochim & Biophys Mol & Cellulaire, F-91400 Orsay, France

[5] CNRS, UPR 3082, Lab Enzymol & Biochim Struct, Ctr Rech Gif, F-91198 Gif Sur Yvette, France

来源：

MOLECULAR MICROBIOLOGY | 2013年 / 88卷 / 01期

关键词：

D O I：

10.1111/mmi.12168

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NprR is a quorum sensor of the RNPP family found in bacteria of the Bacillus cereus group. In association with its cognate peptide NprX, NprR controls the expression of genes essential for survival and sporulation of Bacillus thuringiensis during its necrotrophic development in insects. Here, we report that the nprRnprX genes are not autoregulated and are co-transcribed from a sigma A-dependent promoter (PA) located upstream from nprR. The transcription from PA starts at the onset of the stationary phase and is controlled by two transcriptional regulators: CodY and PlcR. The nutritional repressor CodY represses nprRnprX transcription during the exponential growth phase and the quorum sensor PlcR activates nprRnprX transcription at the onset of stationary phase. We show that nprX is also transcribed independently of nprR from two promoters, PH and PE, dependent on the sporulation-specific sigma factors, sigma H and sigma E respectively. Both promoters ensure nprX transcription during late stationary phase while transcription from PA has decreased. These results show that the activity of the NprRNprX quorum sensing system is tightly co-ordinated to the physiological stage throughout the developmental process of the Bacillus.

引用

页码：48 / 63

页数：16

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