Amniotic fluid induces platelet-neutrophil aggregation and neutrophil activation

被引：10

作者：

Chen, Kuen-Bao ^{[1
]}

Chang, Shih-Sheng ^{[2
,3
]}

Tseng, Yu-Lun ^{[2
,4
]}

Chiu, Tsan-Hung ^{[5
]}

Liao, Cheng-Chun ^{[1
]}

Ho, Ming ^{[5
]}

Huang, Go-Shine ^{[6
]}

Li, Chi-Yuan ^{[1
,2
]}

机构：

[1] China Med Univ Hosp, Dept Anesthesiol, Taichung 40447, Taiwan

[2] China Med Univ, Grad Inst Clin Med Sci, Taichung, Taiwan

[3] China Med Univ Hosp, Dept Med, Div Cardiol, Taichung 40447, Taiwan

[4] China Med Univ Hosp, Dept Psychiat, Taichung 40447, Taiwan

[5] China Med Univ Hosp, Dept Obstet & Gynecol, Taichung 40447, Taiwan

[6] Tri Serv Gen Hosp, Natl Def Med Ctr, Dept Anesthesiol, Taipei, Taiwan

来源：

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | 2013年 / 208卷 / 04期

关键词：

amniotic fluid embolism syndrome (AFES); neutrophil activation; p38 mitogen-activated protein kinase (MAPK); platelet-neutrophil aggregation; P-SELECTIN; SIGNALING PATHWAYS; ENDOTHELIAL-CELLS; CALCIUM; EMBOLISM; INFLAMMATION; MOBILIZATION; MECHANISMS; FILTRATION; ADHESION;

D O I：

10.1016/j.ajog.2013.01.012

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

OBJECTIVE: Amniotic fluid embolism syndrome is a fatal disease in pregnant women. The exact role of platelets and neutrophils in amniotic fluid embolism syndrome is not clear. We examined whether amniotic fluid could affect platelet-neutrophil aggregation and activation and the possible mechanisms. STUDY DESIGN: Blood samples from the pregnant women were pretreated ex vivo with their own amniotic fluid. Flow cytometry was used to measure platelet-neutrophil aggregation and activation. Neutrophil-mediated activity of p38 mitogen-activated protein kinase and extracellular signal-regulated protein kinases 1 and 2 was analyzed by Western blotting. RESULTS: Amniotic fluid significantly induced platelet-neutrophil aggregation, neutrophil CD11b expression, and reactive oxygen species production. Amniotic fluid induced minimal platelet P-selectin expression. The increase of intracellular calcium level of neutrophils and the activity of p38 mitogen-activated protein kinase were enhanced by amniotic fluid stimulation. CONCLUSION: Amniotic fluid was able to induce neutrophil activation and platelet-neutrophil aggregation with minimal effect on platelet activation. These findings may provide a new insight in the understanding of the pathophysiologic condition of amniotic fluid embolism syndrome.

引用

页码：318.e1 / 318.e7

页数：7

共 38 条

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