Identity and public health potential of Cryptosporidium spp. in water buffalo calves in Egypt

被引：46

作者：

Amer, Said ^{[1
,2
]}

Zidan, Shereif ^{[3
]}

Feng, Yaoyu ^{[4
]}

Adamu, Haileeyesus ^{[1
,5
]}

Li, Na ^{[1
,4
]}

Xiao, Lihua ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30329 USA

[2] Kafr El Sheikh Univ, Fac Sci, Dept Zool, Kafe El Sheikh, Egypt

[3] Menoufia Univ, Sadat Branch, Dept Anim Hyg & Zoonoses, Fac Vet Med, Al Menofia, Egypt

[4] E China Univ Sci & Technol, Sch Resources & Environm Engn, Shanghai 200237, Peoples R China

[5] Univ Addis Ababa, Addis Ababa, Ethiopia

来源：

VETERINARY PARASITOLOGY | 2013年 / 191卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Cryptosporidium; Genotype; Subtype; Buffalo; Calves; Egypt; MOLECULAR CHARACTERIZATION; GENETIC CLASSIFICATION; PROTOZOAN PARASITES; BUBALUS-BUBALIS; NATIONAL-PARK; DAIRY CALVES; PREVALENCE; CATTLE; GENOTYPES; SUBTYPES;

D O I：

10.1016/j.vetpar.2012.08.015

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Little is known about the diversity and public health significance of Cryptosporidium species in water buffaloes. In this study, we examined the distribution of Cryptosporidium spp. in water buffalo calves in Egypt. Rectal fecal specimens from 179 calves and 359 adults were screened microscopically for Cryptosporidium oocysts using modified Ziehl-Neelsen stain. Cry ptosporidium spp. in 17 microscopy-positive specimens from calves were genotyped by DNA sequence analysis of the small-subunit rRNA gene, and Cryptosporidium parvum was subtyped by sequence analysis of the 60 kDa glycoprotein gene. Cryptosporidium ryanae was found in 10 specimens and C. parvum in 7 specimens, with the former belonging to the newly identified C. ryanae buffalo variant and the latter belonging to the subtypes IldA20G1 (in 5 specimens) and IlaA15G1R1 (in 2 specimens). The prevailing occurrence of C. ryanae and the subtype family lid of C. parvum and the absence of C. bovis and C. andersoni represent some features of Cryptosporidium transmission in water buffaloes in Egypt. Published by Elsevier B.V.

引用

页码：123 / 127

页数：5

共 40 条

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