EFFECTS OF SMOKING DURING PREGNANCY ON DNA DAMAGE AND ROS LEVEL CONSEQUENCES IN MATERNAL AND NEWBORNS' BLOOD

被引:18
作者
Aydogan, Umit [1 ]
Durmaz, Emre [2 ]
Ercan, Cihangir Mutlu [3 ]
Eken, Ayse [4 ]
Ulutas, Onur Kenan [2 ]
Kavuk, Serkan [1 ]
Gursel, Orhan [5 ]
Alanbay, Ibrahim [3 ]
Akay, Cemal [4 ]
Kurekci, Ahmet Emin [5 ]
Aydin, Ahmet [6 ]
Sayal, Ahmet [4 ]
Saglam, Kenan [1 ]
Cok, Ismet [2 ]
机构
[1] Gulhane Mil Med Acad, Dept Family Med, Ankara, Turkey
[2] Gazi Univ, Fac Pharm, Dept Toxicol, Ankara, Turkey
[3] Gulhane Mil Med Acad, Dept Obstet & Gynecol, Ankara, Turkey
[4] Gulhane Mil Med Acad, Dept Toxicol, Ankara, Turkey
[5] Gulhane Mil Med Acad, Dept Pediat Hematol, Ankara, Turkey
[6] Yeditepe Univ, Fac Pharm, Dept Toxicol, Istanbul, Turkey
来源
ARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU-ARCHIVES OF INDUSTRIAL HYGIENE AND TOXICOLOGY | 2013年 / 64卷 / 01期
关键词
cigarette smoke; cord blood; genotoxicity; oxidative stress; pregnant women; OXIDE SYNTHASE ACTIVITY; SINGLE-STRAND BREAKS; OXIDATIVE STRESS; NITRIC-OXIDE; COMET ASSAY; CHILDHOOD EXPOSURE; CIGARETTE-SMOKING; PASSIVE SMOKING; RISK; ANTIOXIDANTS;
D O I
10.2478/10004-1254-64-2013-2232
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Some of the genotoxic/carcinogenic substances or metabolites in cigarette smoke are capable of passing through the placenta and harming a newborn's health. Smoking is also known as a factor in the formation of oxidative damage and the main mechanism involved in the carcinogenic process. Predetermining this genotoxic risk can be successfully achieved by measuring certain parameters of oxidative stress. The comet assay is considered an important biomarker for the evaluation of genotoxic substances and is effective for detecting DNA damage caused by smoking. This study examined third trimester bloods and the cord blood of 28 actively smoking and 22 non-smoking mothers in terms of DNA damage and oxidative stress parameters. Cu/Zn superoxide dismutase (CuZn-SOD), malondialdehyde (MDA), catalase (CAT), plasma nitrite/nitrates (NO2-/NO3-), selenium-dependent glutathione peroxidase (Se-GPx), Cu, and Zn levels were measured as indicators of oxidative damage. There were no significant increases in DNA damage of the actively smoking pregnant group in comparison with the non-smoking pregnant group, either in the third trimester or cord blood. Oxidative stress parameters of smoker and non-smoker groups were statistically different for MDA (p<0.05), CuZn-SOD (p<0.01), Se-GPx (p<0.05) values while the difference was not significant for NO2-/NO3-, CAT, Zn, and Cu values. The same values were also investigated in cord blood, and only NO2/NO3-(p<0.01), Se-GPx (p<0.01 and CAT (p<0.001) values were found statistically different. Smoking mothers may have been exposed to more oxidative stress than non-smoking mothers.
引用
收藏
页码:35 / 46
页数:12
相关论文
共 62 条
  • [1] Abou-Seif M A, 1996, J Biochem Toxicol, V11, P133, DOI 10.1002/(SICI)1522-7146(1996)11:3<133::AID-JBT5>3.0.CO
  • [2] 2-L
  • [3] Oxidative Stress, Histone Deacetylase and Corticosteroid Resistance in Severe Asthma and COPD
    Adenuga, David
    Rahman, Irfan
    [J]. CURRENT RESPIRATORY MEDICINE REVIEWS, 2007, 3 (01) : 57 - 68
  • [4] Aebi H, 1984, Methods Enzymol, V105, P121
  • [5] Smoking Cessation Early in Pregnancy and Birth Weight, Length, Head Circumference, and Endothelial Nitric Oxide Synthase Activity in Umbilical and Chorionic Vessels An Observational Study of Healthy Singleton Pregnancies
    Andersen, Malene R.
    Simonsen, Ulf
    Uldbjerg, Niels
    Aalkjaer, Christian
    Stender, Steen
    [J]. CIRCULATION, 2009, 119 (06) : 857 - 864
  • [6] Reduced endothelial nitric oxide synthase activity and concentration in fetal umbilical veins from maternal cigarette smokers
    Andersen, MR
    Walker, LR
    Stender, S
    [J]. AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2004, 191 (01) : 346 - 351
  • [7] Increased oxidative/nitrosative stress and decreased antioxidant enzyme activities in prostate cancer
    Arsova-Sarafinovska, Zorica
    Eken, Ayse
    Matevska, Nadica
    Erdem, Onur
    Sayal, Ahmet
    Savaser, Ayhan
    Banev, Saso
    Petrovski, Daniel
    Dzikova, Sonja
    Georgiev, Vladimir
    Sikole, Aleksandar
    Ozgok, Yasar
    Suturkova, Ljubica
    Dimovski, Aleksandar J.
    Aydin, Ahmet
    [J]. CLINICAL BIOCHEMISTRY, 2009, 42 (12) : 1228 - 1235
  • [8] Maternal active or passive smoking causes oxidative stress in cord blood
    Aycicek, Ali
    Ipek, Abdullah
    [J]. EUROPEAN JOURNAL OF PEDIATRICS, 2008, 167 (01) : 81 - 85
  • [9] Oxidative stress and nitric oxide related parameters in type II diabetes mellitus:: effects of glycemic control
    Aydin, A
    Orhan, H
    Sayal, A
    Özata, M
    Sahin, G
    Isimer, A
    [J]. CLINICAL BIOCHEMISTRY, 2001, 34 (01) : 65 - 70
  • [10] Effects of antihypertensive agents, alpha receptor blockers, beta blockers, angiotensin-converting enzyme inhibitors, angiotensin receptor blockers and calcium channel blockers, on oxidative stress
    Baykal, Y
    Yilmaz, MI
    Celik, T
    Gok, F
    Rehber, H
    Akay, C
    Kocar, IH
    [J]. JOURNAL OF HYPERTENSION, 2003, 21 (06) : 1207 - 1211