3D Patterning of cells in Magnetic Scaffolds for Tissue Engineering

被引：80

作者：

Goranov, V ^{[1
,2
]}

Shelyakova, T. ^{[3
]}

De Santis, R. ^{[4
]}

Haranava, Y. ^{[2
]}

Makhaniok, A. ^{[2
]}

Gloria, A. ^{[4
]}

Tampieri, A. ^{[5
]}

Russo, A. ^{[3
]}

Kon, E. ^{[6
,7
,8
]}

Marcacci, M. ^{[6
,7
]}

Ambrosio, L. ^{[4
]}

Dediu, V. A. ^{[1
]}

机构：

[1] CNR ISMN, Inst Nanostruct Mat, Via Gobetti 101, I-40129 Bologna, Italy

[2] BioDevice Syst, Vrsovice 99620 10, Bulharska, Czech Republic

[3] IRCCS Ist Ortoped Rizzoli, Via Barbiano 1-10, I-40136 Bologna, Italy

[4] CNR IPCB, Inst Polymers Composites & Biomat, Vle JF Kennedy 54,Pad 20 Mostra Oltremare, I-80125 Naples, Italy

[5] CNR ISTEC, Inst Sci & Technol Ceram, Via Granarolo 64, I-48018 Faenza, Italy

[6] Humanitas Univ, Dept Biomed Sci, Via Manzoni 113, I-20089 Milan, Italy

[7] Humanitas Clin & Res Ctr, Via Manzoni 56, I-20089 Rozzano Milan, Italy

[8] First Moscow State Med Univ, Sechenov Univ, Moscow, Russia

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

基金：

欧盟地平线“2020”;

关键词：

MESENCHYMAL STEM-CELLS; 3-DIMENSIONAL SCAFFOLDS; BIOMATERIALS; CARTILAGE; CULTURE; NANOPARTICLES; FABRICATION; CONSTRUCTS; SYSTEMS;

D O I：

10.1038/s41598-020-58738-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A three dimensional magnetic patterning of two cell types was realised in vitro inside an additive manufactured magnetic scaffold, as a conceptual precursor for the vascularised tissue. The realisation of separate arrangements of vascular and osteoprogenitor cells, labelled with biocompatible magnetic nanoparticles, was established on the opposite sides of the scaffold fibres under the effect of non-homogeneous magnetic gradients and loading magnetic configuration. The magnetisation of the scaffold amplified the guiding effects by an additional trapping of cells due to short range magnetic forces. The mathematical modelling confirmed the strong enhancement of the magnetic gradients and their particular geometrical distribution near the fibres, defining the preferential cell positioning on the micro-scale. The manipulation of cells inside suitably designed magnetic scaffolds represents a unique solution for the assembling of cellular constructs organised in biologically adequate arrangements.

引用

页数：8

共 52 条

[1] Poly(caprolactone) based magnetic scaffolds for bone tissue engineering [J].

Banobre-Lopez, M. ;

Pineiro-Redondo, Y. ;

De Santis, R. ;

Gloria, A. ;

Ambrosio, L. ;

Tampieri, A. ;

Dediu, V. ;

Rivas, J. .

JOURNAL OF APPLIED PHYSICS, 2011, 109 (07)

[2] A novel route in bone tissue engineering: Magnetic biomimetic scaffolds [J].

Bock, N. ;

Riminucci, A. ;

Dionigi, C. ;

Russo, A. ;

Tampieri, A. ;

Landi, E. ;

Goranov, V. A. ;

Marcacci, M. ;

Dediu, V. .

ACTA BIOMATERIALIA, 2010, 6 (03) :786-796

[3] Photo-patterning of porous hydrogels for tissue engineering [J].

Bryant, Stephanie J. ;

Cuy, Janet L. ;

Hauch, Kip D. ;

Ratner, Buddy D. .

BIOMATERIALS, 2007, 28 (19) :2978-2986

[4] Inkjet printing for materials and devices [J].

Calvert, P .

CHEMISTRY OF MATERIALS, 2001, 13 (10) :3299-3305

[5] Scaffolding in tissue engineering: general approaches and tissue-specific considerations [J].

Chan, B. P. ;

Leong, K. W. .

EUROPEAN SPINE JOURNAL, 2008, 17 (Suppl 4) :S467-S479

[6] Toward delivery of multiple growth factors in tissue engineering [J].

Chen, Fa-Ming ;

Zhang, Min ;

Wu, Zhi-Fen .

BIOMATERIALS, 2010, 31 (24) :6279-6308

[7] Remodeling and homeostasis of the extracellular matrix: implications for fibrotic diseases and cancer [J].

Cox, Thomas R. ;

Erler, Janine T. .

DISEASE MODELS & MECHANISMS, 2011, 4 (02) :165-178

[8] Towards the Design of 3D Fiber-Deposited Poly(ε-caprolactone)/Iron-Doped Hydroxyapatite Nanocomposite Magnetic Scaffolds for Bone Regeneration [J].

De Santis, Roberto ;

Russo, Alessandro ;

Gloria, Antonio ;

D'Amora, Ugo ;

Russo, Teresa ;

Panseri, Silvia ;

Sandri, Monica ;

Tampieri, Anna ;

Marcacci, Maurilio ;

Dediu, Valentin A. ;

Wilde, Colin J. ;

Ambrosio, Luigi .

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2015, 11 (07) :1236-1246

[9] Tissue Engineered Bone Grafts: Biological Requirements, Tissue Culture and Clinical Relevance [J].

Froehlich, Mirjam ;

Grayson, Warren L. ;

Wan, Leo Q. ;

Marolt, Darja ;

Drobnic, Matej ;

Vunjak-Novakovic, Gordana .

CURRENT STEM CELL RESEARCH & THERAPY, 2008, 3 (04) :254-264

[10] Micropatterned cell co-cultures using layer-by-layer deposition of extracellular matrix components [J].

Fukuda, J ;

Khademhosseini, A ;

Yeh, J ;

Eng, G ;

Cheng, JJ ;

Farokhzad, OC ;

Langer, R .

BIOMATERIALS, 2006, 27 (08) :1479-1486

← 1 2 3 4 5 6 →