Hot electron-induced cathodic electrochemiluminescence of rhodamine B at disposable oxide-coated aluminum electrodes

被引：28
作者：
Jiang, QH
Spehar, AM
Håkansson, M
Suomi, J
Ala-Kleme, T
Kulmala, S
机构：
[1] Helsinki Univ Technol, Lab Analyt & Inorgan Chem, FIN-02015 Helsinki, Finland
[2] Turku Univ, Analyt Chem Lab, FIN-20014 Turku, Finland
来源：
ELECTROCHIMICA ACTA | 2006年 / 51卷 / 13期
关键词：
electrochemiluminescence (ECL); tune-resolved detection; rhodamine B; hot electron; tunnel emission; oxide-coated aluminum electrode;
D O I：
10.1016/j.electacta.2005.08.004
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
Rhodamine B (RhB) exhibits strong cathodic electrogeneratcd chemiluminescence (ECL) in aqueous solutions during high-amplitude pulse polarization at thin oxide film-coated aluminum electrodes. This method allows the detection of RhB below nanomolar concentration level and provides linear calibration plots spanning over several orders of magnitude of concentration. In addition, a relatively long ECL lifetime of RhB provides a basis for time-resolved detection. Thus, widely used RhB-based labels can also be suggested to be usable as electrochemiluminescent labels in fully aqueous solutions in bioaffinity assays such as in immunoassays and DNA-probing assays. Support was obtained for the chemiluminescence generation mechanism to be essentially the same as that of radiochemiluminescence in aqueous solution. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2706 / 2714
页数：9
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