Development of human megakaryocytes .1. Hematopoietic progenitors (CD34(+) bone marrow cells) are enriched with megakaryocytes expressing CD4

CD34 is expressed by essentially all human hematopoietic progenitors including cells of the megakaryocyte (MK) lineage. We have previously reported CD4 expression by some human MK (Blood 81:2,664. 1993). To study the role of maturation. ration on CD4 expression by MK, we examined CD34(+) bone marrow cells for their expression of CD41 (GPIIb-GPIIIa) and CD4 with specific monoclonal antibody (MoAb)-fluorochrome conjugates and for DNA polyploidization with propidium iodide or 7-aminoactinomycin D (7-AAD). Surprisingly. MK were at least 20-fold more common in the CD34(+) progenitor pool (similar to 10%) than in the more mature CD34(-) population (similar to 0.5%) of low-density bone marrow cells. CD4 expression correlated with markers of immaturity in that CD4 was enriched among CD34(+) cells, and the proportion of CD4(+) MK declined with increasing ploidy. Almost all CD34(+) polyploid (greater than or equal to 8 N) cells were CD4(+). Despite these correlations with immaturity, CD34(+)CD4(+) MK precursors were unable to produce MK colony-forming units (CFU-MK) when cultured under conditions that supported the growth of CFU-MK from CD34(+)CD4(-) MK lineage cells. MK became polyploid before the loss of either CD34 or CD4 expression. The presence of CD4 on these cells correlates with the onset of endomitotic reduplication and is associated with the loss of the ability of these cells to undergo normal mitotic division. The role of CD4 on immature MK as a differentiation antigen and/or receptor for the human immunodeficiency virus (HIV)-1 virus remains to be determined. (C) 1996 by The American Society of Hematology.