Comparative transcriptome analysis reveals the genetic basis of coat color variation in Pashmina goat

被引：35

作者：

Bhat, Basharat ^{[1
]}

Singh, Ashutosh ^{[1
]}

Iqbal, Zaffar ^{[2
]}

Kaushik, Jai K. ^{[3
]}

Rao, A. R. ^{[5
]}

Ahmad, Syed Mudasir ^{[4
]}

Bhat, Hina ^{[4
]}

Ayaz, Aadil ^{[2
]}

Sheikh, F. D. ^{[2
]}

Kalra, Shalini ^{[3
]}

Shanaz, Syed ^{[2
]}

Mir, Masood Salim ^{[2
]}

Agarwal, Pawan Kumar ^{[6
]}

Mohapatra, Trilochan ^{[6
]}

Ganai, Nazir A. ^{[2
]}

机构：

[1] Shiv Nadar Univ, Dept Life Sci, Gautam Buddha Nagar 201314, UP, India

[2] Sher E Kashmir Univ Agr Sci & Technol Kashmir, Div Anim Genet & Breeding, Shuhama 190016, Jammu & Kashmir, India

[3] Natl Dairy Res Inst, Anim Biotechnol Ctr, Karnal, India

[4] Sher E Kashmir Univ Agr Sci & Technol Kashmir, Div Anim Biotechnol, Shuhama 190016, Jammu & Kashmir, India

[5] Indian Agr Res Inst, Ctr Agr Bioinformat, New Delhi, India

[6] Indian Council Agr Res, New Delhi, India

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

MOUSE SILVER LOCUS; EUMELANIN/PHEOMELANIN SYNTHESIS; PROTEIN FAMILIES; MUTATIONS; PIGMENTATION; PHENOTYPE; DATABASE; MITF; MAPS; ASIP;

D O I：

10.1038/s41598-019-42676-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The genetics of coat color variation remains a classic area. Earlier studies have focused on a limited number of genes involved in color determination; however, the complete set of trait determinants are still not well known. In this study, we used high-throughput sequencing technology to identify and characterize intricate interactions between genes that cause complex coat color variation in Changthangi Pashmina goats, producer of finest and costly commercial animal fiber. We systematically identified differentially expressed mRNAs and lncRNAs from black, brown and white Pashmina goat skin samples by using RNA-sequencing technique. A pairwise comparison of black, white and brown skin samples yielded 2479 significantly dysregulated genes (2422 mRNA and 57 lncRNAs). Differentially expressed genes were enriched in melanin biosynthesis, melanocyte differentiation, developmental pigmentation, melanosome transport activities GO terms. Our analysis suggested the potential role of lncRNAs on color coding mRNAs in cis and trans configuration. We have also developed online data repository as a component of the study to provide a central location for data access, visualization and interpretation accessible through http://pcd.skuaskt.org/.

引用

页数：9

共 38 条

[1] A missense mutation in the agouti signaling protein gene (ASIP) is associated with the no light points coat phenotype in donkeys [J].