Comparative evaluation of indirect and sandwich ELISA for the detection of antibodies to bovine respiratory syncytial virus (BRSV) in dairy cattle

被引：12

作者：

Hazari, S

Panda, HK

Kar, BC

Das, BR

机构：

[1] Orissa Univ Agr & Technol, Dept Bacteriol & Virol, Coll Vet Sci & Anim Husb, Bhubaneswar 751003, Orissa, India

[2] Inst Anim Dis Res, Virol Unit, Cuttack, Orissa, India

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2002年 / 25卷 / 01期

关键词：

BRSV; seroepidermiological/predominance; ELISA;

D O I：

10.1016/S0147-9571(01)00022-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Seroprevalence of bovine respiratory syncytial virus (BRSV) infection in both exotic and crossbred cattle were described. A baculovirus expressed recombinant purified nucleocapsid (N) protein was used in indirect and sandwich ELISA for screening of 499 bovine sera samples from all over the state for the presence of BRSV antibodies. The seroprevalence rate of BRSV was found to be 46.09% through indirect ELISA while it would found to be 65.33% by sandwich ELISA. The result also indicated that exotic breeds were more susceptible to BRSV infection compared to crossbred cattle. A comprehensive analysis on susceptibility to BRSV as regards to various factors like age and sex was also summarized. (C) 2001 Elsevier; Science Ltd. All rights reserved.

引用

页码：59 / 68

页数：10

共 29 条

[1]

BAKER JC, 1986, AM J VET RES, V47, P40

[2] DEVELOPMENT AND APPLICATION OF A MICRONEUTRALIZATION ELISA FOR THE DETECTION OF ANTIBODIES TO BOVINE RESPIRATORY SYNCYTIAL VIRUSES [J].

ELLIS, JA ;

HASSARD, L ;

MORLEY, PS .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1995, 7 (02) :183-189

[3] Severe respiratory disease in dairy cows caused by infection with bovine respiratory syncytial virus [J].

Elvander, M .

VETERINARY RECORD, 1996, 138 (05) :101-105

[4] ENZYME-LINKED-IMMUNOSORBENT-ASSAY USED TO MONITOR SERUM ANTIBODIES TO BOVINE RESPIRATORY-DISEASE VIRUSES [J].

FLORENT, G ;

DEMARNEFFE, C .

VETERINARY MICROBIOLOGY, 1986, 11 (04) :309-317

[5]

FLORENT G, 1995, SCHWEIZER ARCH TIEHE, V127, P661

[6]

GILLETTE KG, 1983, AM J VET RES, V44, P2251

[7]

GOULD EA, 1978, J CLIN MICROBIOL, V8, P233

[8]

Jacobsson S. O., 1989, Svensk Veterinartidning, V41, P641

[9]

Kahrs R. F., 1981, VIRAL DIS CATTLE

[10] EPIDEMIOLOGICAL-STUDY OF BOVINE RESPIRATORY SYNCYTIAL VIRUS-INFECTIONS IN CALVES - INFLUENCE OF MATERNAL ANTIBODIES ON THE OUTCOME OF DISEASE [J].

KIMMAN, TG ;

ZIMMER, GM ;

WESTENBRINK, F ;

MARS, J ;

VANLEEUWEN, E .

VETERINARY RECORD, 1988, 123 (04) :104-109

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