Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus

被引：31

作者：

He, Ming-Liang

Zheng, Bo-Jian

Chen, Ying

Wong, Kin-Ling

Huang, Jian-Dong

Lin, Marie C.

Peng, Ying

Yuen, Kwok Y.

Sung, Joseph J. Y.

Kung, Hsiang-Fu ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Fac Med, Ctr Emerging Infect, Li Kashing Med Inst, Shatin, Hong Kong, Peoples R China

[2] Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China

[3] Univ Hong Kong, Dept Biochem, Hong Kong, Hong Kong, Peoples R China

[4] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China

[5] Sun Yat Sen Univ, Affiliated Hosp 2, Dept Neurol, Guangzhou, Peoples R China

来源：

FEBS LETTERS | 2006年 / 580卷 / 10期

关键词：

SARS-associated coronavirus; viral kinetics; RNA interference; siRNA; inhibition of SCoV reproduction;

D O I：

10.1016/j.febslet.2006.03.066

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SARS-associated coronavirus was identified as the etiological agent of severe acute respiratory syndrome and a large virus pool was identified in wild animals. Virus generates drug resistance through fast mutagenesis and escapes antiviral treatment. siRNAs targeting different genes would be an alternative for overcoming drug resistance. Here, we report effective siRNAs targeting structural genes (i.e., spike, envelope, membrane, and nucleocapsid) and their antiviral kinetics. We also showed the synergistic effects of two siRNAs targeting different functional genes at a very low dose. Our findings may pave a way to develop cost effective siRNA agents for antiviral therapy in the future. (c) 2006 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：2414 / 2420

页数：7

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