Impedance aggregometric analysis of platelet function of apheresis platelet concentrates as a function of storage time

被引：2

作者：

Glas, Michael ^{[1
]}

Bauer, Janine Viola ^{[2
]}

Eichler, Hermann ^{[3
]}

Volk, Thomas ^{[2
]}

机构：

[1] Univ Hosp Bern, Dept Intens Care Med, Inselspital, CH-3010 Bern, Switzerland

[2] Univ Saarland, Dept Anaesthesiol Intens Care & Pain Therapy, Med Ctr, Kirrberger Str, Homburg, Germany

[3] Univ Saarland, Inst Clin Hemostaseol & Transfus Med, Med Ctr, Kirrberger Str, Homburg, Germany

来源：

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION | 2016年 / 76卷 / 08期

关键词：

Blood preservation; electric impedance; platelet aggregation; platelet function tests; plateletpheresis; MULTIPLE ELECTRODE AGGREGOMETRY; WHOLE-BLOOD; ADDITIVE SOLUTIONS; AGGREGATION; PLASMA; TRANSFUSION; METABOLISM; ANTICOAGULANTS; MULTIPLATE(TM); 22-DEGREES-C;

D O I：

10.1080/00365513.2016.1238505

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Multiple electrode (impedance) aggregometry (MEA) allows reliable monitoring of platelet function in whole blood. The aims of the present study were to implement MEA for analyzing aggregation in platelet concentrates and to correlate results with storage time and blood gas analysis (BGA). We investigated the influence of platelet counts, calcium concentrations and agonists on platelet aggregation. Samples of apheresis concentrates up to an age of 12 days were investigated by MEA and BGA. For ASPI- and TRAPtest MEA was reproducible for a platelet count of 400 per 10(-9)L and a calcium concentration of 5mmol L-1. Platelets at the age of 2-4 days yielded steady aggregation. Platelet concentrates exceeding the storage time for transfusion showed steady aggregation up to 10 days, but a significant decline on day 12. Weak correlation was found regarding pCO(2) and MEA as well as regarding glucose concentration and MEA. Our results indicate that MEA is applicable for evaluation of aggregation in stored apheresis concentrates. Prolonged storage seems not to be prejudicial regarding platelet aggregation. Platelet concentrates showed acceptable BGA throughout storage time. Further studies are required to evaluate the application of MEA for quality controls in platelet concentrates.

引用

页码：664 / 670

页数：7

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