LncRNA INHBA-AS1 promotes colorectal cancer cell proliferation by sponging miR-422a to increase AKT1 axis

被引：10
作者：
Lin, H. [1 ,2 ]
Hong, Y. -G. [3 ]
Zhou, J. -D. [3 ]
Gao, X. -H. [3 ]
Yuan, P. -H. [2 ,4 ]
Xin, C. [3 ]
Huang, Z. -P. [5 ]
Zhang, W. [3 ]
Hao, L. -Q. [3 ]
Hou, K. -Z. [1 ,2 ]
机构：
[1] Shidong Hosp, Dept Gen Surg 1, Shanghai, Peoples R China
[2] Univ Shanghai Sci & Technol, Anhui Med Univ, Shanghai, Peoples R China
[3] Second Mil Med Univ, Changhai Hosp, Dept Colorectal Surg, Shanghai, Peoples R China
[4] Shidong Hosp, Dept Operating Room, Shanghai, Peoples R China
[5] Gen Hosp Southern Theatre Command, Dept Hepatobiliary Surg, Guangzhou, Peoples R China
来源：
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES | 2020年 / 24卷 / 19期
关键词：
AKT1; CRC; INHBA-AS1; MiR-422a; Progression; HEPATOCELLULAR-CARCINOMA; INVASION; PROGRESSION; METASTASIS; STEM;
D O I：
10.26355/eurrev_202010_23206
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
OBJECTIVE: In recent years. long non-coding RNAs (lncRNAs) have emerged for regulating the development, as well as progression in colorectal cancer (CRC), which assists in finding new targets for CRC treatment. A previous study indicated that INHBA-AS1 promotes oral squamous cell progression by sponging miR-143-3p. However, the exact function possessed by lncRNA INHBA-AS1 in CRC development remains unclear. PATIENTS AND METHODS: The expression level of INHBA-AS1 in CRC tissues and cell lines was determined by qRT-PCR. The functional role of INHBA-AS1 in CRC was investigated by a series of in vitro assays. RNA immunoprecipitation (RIP), bioinformatics analysis was utilized to explore the potential mechanisms of INHBA-AS1. RESULTS: The present study identified INHBA-AS1 as a kind of IncRNA with high expression in CRC tissues and cells. Functionally. NHBA-AS1 downregulation in CRC cells suppressed CRC cell proliferation as well as colony formability. Mechanistically, INHBA-AS1/miR-422a/AKT1 established the ceRNA network to regulate MMP-2, -7. -9 expressions that participated the modulation of CRC progression. CONCLUSIONS: In summary, LncRNA IN-HBA-AS1 contributes to CRC progression through AKT1 pathway. and provides a new mechanism to regulate CRC development, as well as a potential target for treating CRC.
引用
收藏
页码：9940 / 9948
页数：9
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