Novel polyclonal antibodies as a useful tool for expression studies in amphioxus embryos

被引:10
作者
Bozzo, Matteo [1 ]
Pergner, Jiri [1 ]
Kozmik, Zbynek [1 ]
Kozmikova, Iryna [1 ]
机构
[1] Czech Acad Sci, Inst Mol Genet, Lab Transcript Regulat, Prague, Czech Republic
关键词
Branchiostoma; amphioxus; antibody; expression pattern; LIM-HOMEOBOX GENE; BRANCHIOSTOMA-LANCEOLATUM; DEVELOPMENTAL EXPRESSION; ASYMMETRON-LUCAYANUM; BETA-CATENIN; EVOLUTION; INSIGHTS; LANCELET; GENOME; VERTEBRATES;
D O I
10.1387/ijdb.170259ik
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cephalochordates, commonly called amphioxus or lancelets, are widely regarded as a useful proxy for the chordate ancestor. In recent decades, expression patterns of important developmental genes have been used extensively to infer homologies between cephalochordate and vertebrate embryos. Such comparisons provided important insight into cephalochordate biology and the origin of vertebrate traits. Most of the developmental expression data are collected using whole-mount in situ hybridization that allows the distributions of specific transcripts to be detected in fixed embryos. Here, we describe an experimental pipeline for production of small amounts of functional antibodies directed against amphioxus antigens for use in immunohistochemical labelling. In this pilot study, we generated antibodies against beta-catenin and the transcription factors FoxA, Lhx1, Lhx3 and Pax6. We demonstrate the usefulness of antibodies by performing immunostainings on fixed specimens of B. lanceolatum and B. floridae. We anticipate that amphioxus-specific antibodies will provide a useful tool for high-resolution labelling of individual cells within the embryo and for determining the subcellular localization of the corresponding proteins.
引用
收藏
页码:793 / 800
页数:8
相关论文
共 29 条
[1]   A single three-dimensional chromatin compartment in amphioxus indicates a stepwise evolution of vertebrate Hox bimodal regulation [J].
Acemel, Rafael D. ;
Tena, Juan J. ;
Irastorza-Azcarate, Ibai ;
Marletaz, Ferdinand ;
Gomez-Marin, Carlos ;
de la Calle-Mustienes, Elisa ;
Bertrand, Stephanie ;
Diaz, Sergio G. ;
Aldea, Daniel ;
Aury, Jean-Marc ;
Mangenot, Sophie ;
Holland, Peter W. H. ;
Devos, Damien P. ;
Maeso, Ignacio ;
Escriva, Hector ;
Luis Gomez-Skarmeta, Jose .
NATURE GENETICS, 2016, 48 (03) :336-341
[2]   Evolutionary crossroads in developmental biology: amphioxus [J].
Bertrand, Stephanie ;
Escriva, Hector .
DEVELOPMENT, 2011, 138 (22) :4819-4830
[3]   Insights into spawning behavior and development of the European amphioxus (Branchiostoma lanceolatum) [J].
Fuentes, Michael ;
Benito, Elia ;
Bertrand, Stephanie ;
Paris, Mathilde ;
Mignardot, Aurelie ;
Godoy, Laura ;
Jimenez-Delgado, Senda ;
Oliveri, Diana ;
Candiani, Simona ;
Hirsinger, Estelle ;
D'Aniello, Salvatore ;
Pascual-Anaya, Juan ;
Maeso, Ignacio ;
Pestarino, Mario ;
Vernier, Philippe ;
Nicolas, Jean-Francois ;
Schubert, Michael ;
Laudet, Vincent ;
Geneviere, Anne Marie ;
Albalat, Ricard ;
Fernandez, Jordi Garcia ;
Holland, Nicholas D. ;
Escriva, Hector .
JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION, 2007, 308B (04) :484-493
[4]  
Glardon S, 1998, DEVELOPMENT, V125, P2701
[5]   The amphioxus genome illuminates vertebrate origins and cephalochordate biology [J].
Holland, Linda Z. ;
Albalat, Ricard ;
Azumi, Kaoru ;
Benito-Gutierrez, Elia ;
Blow, Matthew J. ;
Bronner-Fraser, Marianne ;
Brunet, Frederic ;
Butts, Thomas ;
Candiani, Simona ;
Dishaw, Larry J. ;
Ferrier, David E. K. ;
Garcia-Fernandez, Jordi ;
Gibson-Brown, Jeremy J. ;
Gissi, Carmela ;
Godzik, Adam ;
Hallbook, Finn ;
Hirose, Dan ;
Hosomichi, Kazuyoshi ;
Ikuta, Tetsuro ;
Inoko, Hidetoshi ;
Kasahara, Masanori ;
Kasamatsu, Jun ;
Kawashima, Takeshi ;
Kimura, Ayuko ;
Kobayashi, Masaaki ;
Kozmik, Zbynek ;
Kubokawa, Kaoru ;
Laudet, Vincent ;
Litman, Gary W. ;
McHardy, Alice C. ;
Meulemans, Daniel ;
Nonaka, Masaru ;
Olinski, Robert P. ;
Pancer, Zeev ;
Pennacchio, Len A. ;
Pestarino, Mario ;
Rast, Jonathan P. ;
Rigoutsos, Isidore ;
Robinson-Rechavi, Marc ;
Roch, Graeme ;
Saiga, Hidetoshi ;
Sasakura, Yasunori ;
Satake, Masanobu ;
Satou, Yutaka ;
Schubert, Michael ;
Sherwood, Nancy ;
Shiina, Takashi ;
Takatori, Naohito ;
Tello, Javier ;
Vopalensky, Pavel .
GENOME RESEARCH, 2008, 18 (07) :1100-1111
[6]   Nuclear β-catenin promotes non-neural ectoderm and posterior cell fates in amphioxus embryos [J].
Holland, LZ ;
Panfilio, KA ;
Chastain, R ;
Schubert, M ;
Holland, ND .
DEVELOPMENTAL DYNAMICS, 2005, 233 (04) :1430-1443
[7]   Laboratory Spawning and Development of the Bahama Lancelet, Asymmetron lucayanum (Cephalochordata): Fertilization Through Feeding Larvae [J].
Holland, Nicholas D. ;
Holland, Linda Z. .
BIOLOGICAL BULLETIN, 2010, 219 (02) :132-141
[8]   Decelerated genome evolution in modern vertebrates revealed by analysis of multiple lancelet genomes [J].
Huang, Shengfeng ;
Chen, Zelin ;
Yan, Xinyu ;
Yu, Ting ;
Huang, Guangrui ;
Yan, Qingyu ;
Pontarotti, Pierre Antoine ;
Zhao, Hongchen ;
Li, Jie ;
Yang, Ping ;
Wang, Ruihua ;
Li, Rui ;
Tao, Xin ;
Deng, Ting ;
Wang, Yiquan ;
Li, Guang ;
Zhang, Qiujin ;
Zhou, Sisi ;
You, Leiming ;
Yuan, Shaochun ;
Fu, Yonggui ;
Wu, Fenfang ;
Dong, Meiling ;
Chen, Shangwu ;
Xu, Anlong .
NATURE COMMUNICATIONS, 2014, 5 :5896
[9]   Evolutionary history of the extant amphioxus lineage with shallow-branching diversification [J].
Igawa, Takeshi ;
Nozawa, Masafumi ;
Suzuki, Daichi G. ;
Reimer, James D. ;
Morov, Arseniy R. ;
Wang, Yiquan ;
Henmi, Yasuhisa ;
Yasui, Kinya .
SCIENTIFIC REPORTS, 2017, 7
[10]   Pax-Six-Eya-Dach network during amphioxus development:: Conservation in vitro but context specificity in vivo [J].
Kozmik, Zbynek ;
Holland, Nicholas D. ;
Kreslova, Jana ;
Oliveri, Diana ;
Schubert, Michael ;
Jonasova, Kristyna ;
Holland, Linda Z. ;
Pestarino, Mario ;
Benes, Vladimir ;
Candiani, Simona .
DEVELOPMENTAL BIOLOGY, 2007, 306 (01) :143-159