The manner in which DNA is packaged with TFAM has an impact on transcription activation and inhibition

被引：9

作者：

Furukawa, Ryo ^{[1
]}

Yamada, Yuma ^{[1
]}

Matsushima, Yuichi ^{[2
]}

Goto, Yu-ichi ^{[2
]}

Harashima, Hideyoshi ^{[1
]}

机构：

[1] Hokkaido Univ, Fac Pharmaceut Sci, Lab Mol Design Pharmaceut, Kita Ku, Sapporo, Hokkaido 0600812, Japan

[2] Natl Ctr Neurol & Psychiat, Natl Inst Neurosci, Dept Mental Retardat & Birth Defect Res, Kodaira, Tokyo 1878502, Japan

来源：

FEBS OPEN BIO | 2012年 / 2卷

关键词：

Mitochondrial transcription factor A (TFAM); DNA packaging; Mitochondrial DNA (mtDNA); Mitochondrial transcription;

D O I：

10.1016/j.fob.2012.06.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

For successful mitochondrial transgene expression, an optimal packaging exogenous DNA is an important issue. We report herein on the effects of DNA packaged with mitochondrial transcription factor A (TFAM), which packages mitochondrial DNA (mtDNA), on the transcription process. Our initial findings indicated that the transcription of the TFAM/DNA complex was activated, when the complex was formed at an optimal ratio. We also found that TFAM has a significant advantage over protamine, a nuclear DNA packaging protein, from the viewpoint of transcription efficiency. This result indicates that TFAM can be useful packaging protein for exogenous DNA to achieve mitochondrial transgene expression. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：145 / 150

页数：6

共 33 条

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