Eleven new highly polymorphic microsatellite loci in the yellow croaker, Pseudosciaena crocea

被引：11

作者：

An, HS

Cho, KC

Park, JY

机构：

[1] Natl Fisheries Res & Dev Inst, Shellfish Genet & Breeding Res Ctr, Cheju 695835, South Korea

[2] Natl Fisheries Res & Dev Inst, W Sea Fisheries Res Inst, Cheju 695835, South Korea

[3] Natl Fisheries Res & Dev Inst, Biotechnol Res Ctr, Cheju 695835, South Korea

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 04期

关键词：

genetic markers; microsatellites; Pseudosciaena crocea; yellow croaker;

D O I：

10.1111/j.1471-8286.2005.01097.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed 11 new microsatellite markers in Pseudosciaena crocea by screening an enriched genomic library using nonradioactive polymerase chain reaction (PCR) techniques. All loci were found to be polymorphic with an average of 14.9 alleles per locus (range four to 30). The mean observed and expected heterozygosities were 0.86 (range 0.57-1.00) and 0.90 (range 0.62-0.98), respectively. Four loci showed significant Hardy-Weinberg disequilibrium. The high variabilities revealed in this study suggest that these microsatellite loci should provide useful markers for population genetic studies of P. crocea.

引用

页码：866 / 868

页数：3

共 9 条

[1] Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea [J].