Knockdown of CD146 reduces the migration and proliferation of human endothelial cells

被引：65
作者：
Kang, Yanyong
Wang, Fengcai
Feng, Jing
Yang, Dongling
Yang, Xu
Yan, Xiyun [1 ]
机构：
[1] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
[2] Anyang Teachers Coll, Dept Chem, Anyang 455000, Peoples R China
[3] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China
来源：
CELL RESEARCH | 2006年 / 16卷 / 03期
关键词：
small interfering RNA; adhesion molecule CD146; vascular endothelial cell;
D O I：
10.1038/sj.cr.7310039
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Our previous study has demonstrated that CD 146 molecule is a biomarker on vascular endothelium, which is involved in angiogenesis and tumor growth. However the mechanism behind is not clear. Here we have for the first time developed a novel CD 146 blockade system using CD 146 siRNA to study its function on endothelial cells. Our data showed that CD146 siRNA specifically blocked the expression of CD146 on both mRNA and protein levels, leading to the significant suppression of HUVEC proliferation, adhesion and migration. These results demonstrate that CD146 plays a key role in vascular endothelial cell activity and angiogenesis, and CD146 siRNA can be used as a new inhibitor for anti-angiogenesis therapy.
引用
收藏
页码：313 / 318
页数：6
相关论文
共 22 条
[1]   Identification of the S-Endo 1 endothelial-associated antigen [J].
Bardin, N ;
Frances, V ;
Lesaule, G ;
Horschowski, N ;
George, F ;
Sampol, J .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 218 (01) :210-216
[2]   REQUIREMENT OF VASCULAR INTEGRIN ALPHA(V)BETA(3) FOR ANGIOGENESIS [J].
BROOKS, PC ;
CLARK, RAF ;
CHERESH, DA .
SCIENCE, 1994, 264 (5158) :569-571
[3]   Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems [J].
Caplen, NJ ;
Parrish, S ;
Imani, F ;
Fire, A ;
Morgan, RA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) :9742-9747
[4]   Angiogenesis in cancer and other diseases [J].
Carmeliet, P ;
Jain, RK .
NATURE, 2000, 407 (6801) :249-257
[5]   Angiogenesis in health and disease [J].
Carmeliet, P .
NATURE MEDICINE, 2003, 9 (06) :653-660
[6]   Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].
Elbashir, SM ;
Harborth, J ;
Lendeckel, W ;
Yalcin, A ;
Weber, K ;
Tuschl, T .
NATURE, 2001, 411 (6836) :494-498
[7]   Discovery and development of bevacizumab, an anti-VEGF antibody for treating cancer [J].
Ferrara, N ;
Hillan, KJ ;
Gerber, HP ;
Novotny, W .
NATURE REVIEWS DRUG DISCOVERY, 2004, 3 (05) :391-400
[8]   Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].
Fire, A ;
Xu, SQ ;
Montgomery, MK ;
Kostas, SA ;
Driver, SE ;
Mello, CC .
NATURE, 1998, 391 (6669) :806-811
[9]  
FOLKMAN J, 1971, NEW ENGL J MED, V285, P1182
[10]   ANGIOGENESIS IN CANCER, VASCULAR, RHEUMATOID AND OTHER DISEASE [J].
FOLKMAN, J .
NATURE MEDICINE, 1995, 1 (01) :27-31
123