Positioning of two alpha subunit carboxy-terminal domains of RNA polymerase at promoters by two transcription factors

被引：77
|
作者：
Murakami, K
Owens, JT
Belyaeva, TA
Meares, CF
Busby, SJW
Ishihama, A
机构：
[1] NATL INST GENET,DEPT MOL GENET,MISHIMA,SHIZUOKA 411,JAPAN
[2] GRAD UNIV ADV STUDIES,SCH LIFE SCI,MISHIMA,SHIZUOKA 411,JAPAN
[3] UNIV CALIF DAVIS,DEPT CHEM,DAVIS,CA 95616
[4] UNIV BIRMINGHAM,SCH BIOCHEM,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 21期
基金：
英国惠康基金;
关键词：
protein-protein contact; protein-DNA contact; chemical nuclease; transcription regulation;
D O I：
10.1073/pnas.94.21.11274
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Interactions between the cAMP receptor protein (CRP) and the carboxy-terminal regulatory domain (CTD) of Escherichia coli RNA polymerase alpha subunit were analyzed at promoters carrying tandem DNA sites for CRP binding using a chemical nuclease covalently attached to alpha. Each CRP dimer was found to direct the positioning of one of the two alpha subunit CTDs. Thus, the function of RNA polymerase may be subject to regulation through protein-protein interactions between the two (alpha subunits and two different species of transcription factors.
引用
收藏
页码：11274 / 11278
页数：5
相关论文
共 50 条
  • [1] THE CARBOXY-TERMINAL DOMAIN OF THE RNA-POLYMERASE II LARGEST SUBUNIT CAN FUNCTION AS AN ACTIVATOR OF TRANSCRIPTION
    XIAO, H
    LIS, JT
    FRIESEN, JD
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1993, : 99 - 99
  • [2] Role of RNA polymerase II carboxy-terminal domain in coordinating transcription with RNA processing
    McCracken, S
    Rosonina, E
    Fong, N
    Sikes, M
    Beyer, A
    O'Hare, K
    Shuman, S
    Bentley, D
    COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1998, 63 : 301 - 309
  • [3] YEAST RNA-POLYMERASE II - FUNCTIONAL REDUNDANCY OF CARBOXY-TERMINAL DOMAIN OF THE LARGEST SUBUNIT IN INVITRO TRANSCRIPTION
    PATTURAJAN, M
    ANTONY, A
    RAO, GR
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1993, : 87 - 87
  • [4] The carboxy-terminal coiled-coil of the RNA polymerase β′-subunit is the main binding site for Gre factors
    Vassylyeva, Marina N.
    Svetlov, Vladimir
    Dearborn, Altaira D.
    Klyuyev, Sergiy
    Artsimovitch, Irina
    Vassylyev, Dmitry G.
    EMBO REPORTS, 2007, 8 (11) : 1038 - 1043
  • [5] The code and beyond: transcription regulation by the RNA polymerase II carboxy-terminal domain
    Kevin M. Harlen
    L. Stirling Churchman
    Nature Reviews Molecular Cell Biology, 2017, 18 : 263 - 273
  • [6] The code and beyond: transcription regulation by the RNA polymerase II carboxy-terminal domain
    Harlen, Kevin M.
    Churchman, L. Stirling
    NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2017, 18 (04) : 263 - 273
  • [7] Recognition of RNA polymerase II carboxy-terminal domain by 3′-RNA-processing factors
    Anton Meinhart
    Patrick Cramer
    Nature, 2004, 430 : 223 - 226
  • [8] Recognition of RNA polymerase II carboxy-terminal domain by 3′-RNA-processing factors
    Meinhart, A
    Cramer, P
    NATURE, 2004, 430 (6996) : 223 - 226
  • [9] Requirement for two copies of RNA polymerase α subunit C-terminal domain for synergistic transcription activation at complex bacterial promoters
    Lloyd, GS
    Niu, W
    Tebbutt, J
    Ebright, RH
    Busby, SJW
    GENES & DEVELOPMENT, 2002, 16 (19) : 2557 - 2565
  • [10] RNA polymerase II carboxy-terminal domain with multiple connections
    Cho, Eun-Jung
    EXPERIMENTAL AND MOLECULAR MEDICINE, 2007, 39 (03): : 247 - 254
12345