Functional characterization of ex vivo blood myeloid and plasmacytoid dendritic cells after infection with dengue virus

被引：75

作者：

Sun, Peifang ^{[1
]}

Fernandez, Stefan ^{[2
]}

Marovich, Mary A. ^{[2
]}

Palmer, Dupeh R. ^{[2
]}

Celluzzi, Christina M. ^{[3
]}

Boonnak, Kobporn ^{[2
]}

Liang, Zhaodong ^{[1
]}

Subramanian, Hemavathy ^{[1
]}

Porter, Kevin R. ^{[1
]}

Sun, Wellington ^{[2
]}

Burgess, Timothy H. ^{[1
]}

机构：

[1] USN, Med Res Ctr, Silver Spring, MD 20910 USA

[2] Walter Reed Army Inst Res, Silver Spring, MD USA

[3] Univ Maryland, Sch Med, Baltimore, MD 21201 USA

来源：

VIROLOGY | 2009年 / 383卷 / 02期

关键词：

Dengue virus; Viral infection; Dendritic cell subset; Cytokine; Toll-like receptor; DOUBLE-STRANDED-RNA; TOLL-LIKE RECEPTOR-3; INTERFERON-PRODUCING CELLS; PROTEIN-KINASE-R; DC-SIGN; ACTIVATION; RECOGNITION; SUBSETS; DISEASE; TLR7;

D O I：

10.1016/j.virol.2008.10.022

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Myeloid and plasmacytoid dendritic cells (mDC and pDC) are naturally distinctive subsets. We exposed both subsets to dengue virus (DV) in vitro and investigated their functional characteristics. High levels of DV replication in mDC were found to correlate with DC-SIGN expression. Production of inflammatory cytokines by mDC increased gradually after DV-infection, which was dependent on DV replication. Co-stimulatory markers were upregulated on mDC upon DV-infection. On the contrary, lower levels of DV-replication were observed in pDC, but the cytokine production in pDC was quicker and stronger. This cytokine response was not dependent on viral replication, but dependent on cell endosomal activity and TLR7, and could be also induced by purified DV genome RNA. These results clearly suggested functional differences between mDC and pDC in response to DV infection. Additionally, the TLR7-mediated recognition of DV RNA may be involved in pDC functional activation. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：207 / 215

页数：9

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