Enhancement of laccase production and biotechnological application of laccase pleurotus florida

Laccases are oxidoreductases belonging to the multinuclear copper-containing oxidases; they catalyze the monoelectronic oxidation of substrates at the expense of molecular oxygen. Laccases are multicopper oxidation of a wide range of inorganic substances coupled with one four electron reduction of oxygen to water. In this work laccase was produced from Pleurotus florida by using two different production media (media I and II) and production media II was found to be the best among two. All the laccase enzymes are extracellular glycoproteins and many of these enzymes show considerable heterogeneity after purification, so the enzyme is purified and parameters such as optimum day substrate concentration are characterized. Carbon and nitrogen sources in the medium play an important role in ligninolytic enzyme production, so effective carbon and nitrogen sources for optimum production of laccase was also found out. The molecular mass of lacasse isozymes varies from 50 to 100KDa. The molecular mass of partially purified lacasse was determined by using SDS-PAGE technique. Number of industrial applications for laccases has been proposed. In this study biopulping of saw dust using isolated laccase was carried out and kappa number of bleached pulp was obtained.