Ultrastructural observations of human epidermal melanocytes cultured on polyethylene terephthalate film

被引：4
作者：
Huang, Qingxia [1 ]
Liang, Weiquan [1 ]
Xu, Dandan [2 ]
Zhou, Yuanjun [1 ]
Wang, Tengbin [1 ]
Liang, Yan [1 ]
Li, Weiqiu [2 ]
Zhong, Yaotang [1 ]
Chen, Weiying [2 ]
机构：
[1] Shantou Univ, Coll Med, Shantou 515041, Guangdong, Peoples R China
[2] Shantou Univ, Coll Med, Expt Teaching Ctr Preclin Med, Shantou 515041, Guangdong, Peoples R China
来源：
MICRON | 2013年 / 48卷
关键词：
Melanocyte; Melanosome; Transfer; Ultrastructure; PET film; MELANIN TRANSFER; MELANOSOMES; CELLS; KERATINOCYTES; BIOGENESIS; FILOPODIA; LIGHT; MODEL;
D O I：
10.1016/j.micron.2013.02.008
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Objective: To assess the application of polyethylene terephthalate film as a supporting material of cultured human epidermal melanocytes and to observe the ultrastructure of human epidermal melanocytes in vitro. Methods: Human epidermal melanocytes were isolated from 7- to 14-years old children foreskins and were cultured in M254 culture medium containing human melanocyte growth supplement. Cultured melanocytes were purified via a differential trypsinization method. Purified melanocytes were cultured on a film and prepared for transmission electron microscopy. Results: In comparing with the cellular supporting materials polyvinylidene chloride and cellophane, human epidermal melanocytes only attached and grew on polyethylene terephthalate film. Melanocytes grown on polyethylene terephthalate film maintained an intact shape, and retained special ultrastructures characteristic of melanocytes such as dendrites. Conclusions: Polyethylene terephthalate film proved to be an ideal cellular supporting material for the cultivation of human epidermal melanocytes. Ultrastructure of melanocytes showed melanosomes are transferred both from the tip and middle section of dendrites. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：49 / 53
页数：5
相关论文
共 50 条
  • [21] SUBCELLULAR FRACTIONATION OF CULTURED NORMAL HUMAN MELANOCYTES - NEW INSIGHTS INTO THE RELATIONSHIP OF MELANOSOMES WITH LYSOSOMES AND PEROXISOMES
    SMIT, NPM
    VANROERMUND, CWT
    AERTS, HMFG
    HEIKOOP, JC
    VANDENBERG, M
    PAVEL, S
    WANDERS, RJA
    BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1181 (01) : 1 - 6
  • [22] Cultured human dermal papilla cells secrete a chemotactic factor for melanocytes
    Ideta, R
    Soma, T
    Tsunenaga, M
    Ifuku, O
    JOURNAL OF DERMATOLOGICAL SCIENCE, 2002, 28 (01) : 48 - 59
  • [23] Effect of chromium coating thickness on surface adhesion of polyethylene terephthalate optical film
    Li, Yue
    Yang, Guang
    Deng, Dingrong
    Zhang, Yuzhou
    SURFACES AND INTERFACES, 2021, 26
  • [24] ULTRASTRUCTURAL-STUDY OF CULTURED HUMAN MENINGIOMA CELLS
    KAWAMOTO, K
    HERZ, F
    KAJIKAWA, H
    HIRANO, A
    ACTA NEUROPATHOLOGICA, 1979, 46 (1-2) : 11 - 15
  • [25] In vitro proliferation of human epidermal melanocytes biopsied from multiple anatomical sites
    Yang, Hui-Ju
    Yang, Kuo-Chia
    Wang, Yu-Fen
    Yang, Yuan-Ting
    Ko, Jiunn-Liang
    JOURNAL OF COSMETIC DERMATOLOGY, 2020, 19 (11) : 3077 - 3082
  • [26] Cultured human melanocytes express functional Toll-like receptors 2-4, 7 and 9
    Yu, Ning
    Zhang, Shujie
    Zuo, Fuguo
    Kang, Kefei
    Guan, Ming
    Xiang, Leihong
    JOURNAL OF DERMATOLOGICAL SCIENCE, 2009, 56 (02) : 113 - 120
  • [27] Functional vanilloid receptors in cultured normal human epidermal keratinocytes
    Inoue, K
    Koizumi, S
    Fuziwara, S
    Denda, S
    Inoue, K
    Denda, M
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 291 (01) : 124 - 129
  • [28] Human melanocytes grown in epidermal equivalents transfer their melanin to follicular outer root sheath keratinocytes
    Limat, A
    Salomon, D
    Carraux, P
    Saurat, JH
    Hunziker, T
    ARCHIVES OF DERMATOLOGICAL RESEARCH, 1999, 291 (06) : 325 - 332
  • [29] Imatinib inhibits migration of normal human epidermal melanocytes and suppress the phosphorylation of C-kit
    Xia, Jian-xin
    Li, Fu-qiu
    Urabe, Kazunori
    Wang, Jin-feng
    Furue, Masutaka
    BANGLADESH JOURNAL OF PHARMACOLOGY, 2012, 7 (02) : 100 - 103
  • [30] Human melanocytes grown in epidermal equivalents transfer their melanin to follicular outer root sheath keratinocytes
    A. Limat
    Denis Salomon
    Pierre Carraux
    Jean-Hilaire Saurat
    Thomas Hunziker
    Archives of Dermatological Research, 1999, 291 : 325 - 332
12345