Isolation and characterization of genes encoding GRF transcription factors and GIF transcriptional coactivators in Maize (Zea mays L.)

被引：131

作者：

Zhang, Deng-Feng ^{[1
]}

Li, Bo ^{[1
]}

Jia, Guan-Qing ^{[1
]}

Zhang, Ti-Fu ^{[1
]}

Dai, Jing-Rui ^{[1
]}

Li, Jian-Sheng ^{[1
]}

Wang, Shou-Cai ^{[1
]}

机构：

[1] China Agr Univ, Natl Maize Improvement Ctr China, Minist Agr, Dept Genet & Plant Breeding,Key Lab Crop Genom &, Beijing 100193, Peoples R China

来源：

PLANT SCIENCE | 2008年 / 175卷 / 06期

基金：

国家高技术研究发展计划(863计划);

关键词：

ZmGRF; ZmGIF; Transcription factor; Expression; Transgenic lines; Maize;

D O I：

10.1016/j.plantsci.2008.08.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been a long-held view that regulation of gene expression in eukaryotes occurs predominantly at the transcriptional level. GRF (Growth-regulating factor) and GIF (GRF-interacting factor) proteins are putative novel transcriptional regulators and coactivators which form protein complexes and are involved in regulating the growth and development of certain tissues and/or organs in plants. In this study, 14 homologs of ZmGRF genes and 3 homologs of ZmGIF genes from maize (Zea mays L.) were identified and characterized. Fourteen GRF proteins were divided into three subfamilies. ZmGRF genes of C subfamily were mainly expressed in immature ears. Furthermore, overexpression of both ZmGRF11-ZmGIF2 and ZmGRF2-ZmGIF3 resulted in delayed bolting but accelerating the growth of inflorescence stem when compared to wild-type Arabidopsis thaliana. As a result, we postulated that these four genes which expressed preferentially in immature ear tissues in maize might play important roles in the growth and development of ears and might contribute to improving corn yields. (c) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：809 / 817

页数：9

共 13 条

[1] Dendrite development regulated by CREST, a calcium-regulated transcriptional activator [J].

Aizawa, H ;

Hu, SC ;

Bobb, K ;

Balakrishnan, K ;

Ince, G ;

Gurevich, I ;

Cowan, M ;

Ghosh, A .

SCIENCE, 2004, 303 (5655) :197-202

[2]

Bush DS, 1996, PLANTA, V199, P89, DOI 10.1007/BF00196885

[3] Whole genome analysis of the OsGRF gene family encoding plant-specific putative transcription activators in rice (Oryza sativa L.) [J].

Choi, D ;

Kim, JH ;

Kende, H .

PLANT AND CELL PHYSIOLOGY, 2004, 45 (07) :897-904

[4] Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].

Clough, SJ ;

Bent, AF .

PLANT JOURNAL, 1998, 16 (06) :735-743

[5] Polarized growth: maintaining focus on the tip [J].

Cole, Rex A. ;

Fowler, John E. .

CURRENT OPINION IN PLANT BIOLOGY, 2006, 9 (06) :579-588

[6] The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana [J].

Horiguchi, G ;

Kim, GT ;

Tsukaya, H .

PLANT JOURNAL, 2005, 43 (01) :68-78

[7] Comparison of reference genes for quantitative real-time polymerase chain reaction analysis of gene expression [J].

Iskandar, HM ;

Simpson, RS ;

Casu, RE ;

Bonnett, GD ;

Maclean, DJ ;

Manners, JM .

PLANT MOLECULAR BIOLOGY REPORTER, 2004, 22 (04) :325-337

[8] A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis [J].

Kim, JH ;

Kende, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (36) :13374-13379

[9] The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis [J].

Kim, JH ;

Choi, DS ;

Kende, H .

PLANT JOURNAL, 2003, 36 (01) :94-104

[10] Initiation of translation in prokaryotes and eukaryotes [J].

Kozak, M .

GENE, 1999, 234 (02) :187-208

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