Metal-organic frameworks-based biosensor for sequence-specific recognition of double-stranded DNA

被引：106

作者：

Chen, Lifen ^{[1
]}

Zheng, Hanye ^{[2
]}

Zhu, Xi ^{[2
]}

Lin, Zhenyu ^{[2
]}

Guo, Longhua ^{[2
]}

Qiu, Bin ^{[2
]}

Chen, Guonan ^{[2
]}

Chen, Zhong-Ning ^{[1
]}

机构：

[1] Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fuzhou 350002, Peoples R China

[2] Fuzhou Univ, Dept Chem, Fujian Prov Lab Anal & Detect Technol Food Safety, MOE Key Lab Anal & Detect Food Safety, Fuzhou 350002, Peoples R China

来源：

ANALYST | 2013年 / 138卷 / 12期

关键词：

COORDINATION POLYMER; RNASE-H; INITIATION; LIGAND;

D O I：

10.1039/c3an00426k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple, cost-efficient, sensitive and selective fluorescence sensor is developed for sequence-specific recognition of duplex DNA (ds-DNA) in vitro using metal-organic framework (MOF) as the sensing platform. N,N-Bis(2-hydroxy-ethyl) dithiooxamidatocopper((II)) (H(2)dtoaCu) was chosen as the example MOF, because it strongly chemisorbs the dye-labeled probe TFO (triplex-forming oligonucleotide), and quenches fluorescence from the dye. In the presence of target ds-DNA (the PPT of HIV RNA, a 16-bp ds-DNA sequence), the TFO could interact with the major groove in ds-DNA (via Hoogsteen hydrogen bonding) to form a rigid triplex structure, resulting in fluorescence recovery. The enhanced fluorescence signal has a relationship with the ds-DNA concentration, the detection limit is as low as 1.3 nmol L-1 (S/N = 3) with good selectivity, which is lower than that based on a graphene oxide platform and electrochemical-DNA sensor.

引用

页码：3490 / 3493

页数：4

共 25 条

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